LOCUS       V001617                 5369 bp    DNA     circular SYN 13-MAY-2021
DEFINITION  Exported.
ACCESSION   V001617
VERSION     V001617
KEYWORDS    DsRed-Rab5 WT.
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 5369)
  AUTHORS   Sharma DK, Choudhury A, Singh RD, Wheatley CL, Marks DL, Pagano RE
  TITLE     Glycosphingolipids internalized via caveolar-related endocytosis
            rapidly merge with the clathrin pathway in early endosomes and form
            microdomains for recycling.
  JOURNAL   J Biol Chem. 2003 Feb 28. 278(9):7564-72.
   PUBMED   12482757
REFERENCE   2  (bases 1 to 5369)
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 5369)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol
            Chem. 2003 Feb 28. 278(9):7564-72."
            SGRef: number: 2; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5369
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        16..319
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        320..523
                     /label="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     CDS             568..1242
                     /label="DsRed2"
                     /note="improved tetrameric variant of DsRed fluorescent
                     protein"
     regulatory      1249..1258
                     /label="Kozak sequence"
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1261..1287
                     /label="HA"
                     /note="HA (human influenza hemagglutinin) epitope tag"
     CDS             1291..1932
                     /gene="RAB5A"
                     /label="Ras-related protein Rab-5A"
                     /note="Ras-related protein Rab-5A from Homo sapiens.
                     Accession#: P20339"
     misc_feature    1936..1988
                     /label="MCS"
                     /note="multiple cloning site"
     polyA_signal    2112..2233
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(2240..2695)
                     /direction=LEFT
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        2722..2826
                     /label="AmpR promoter"
     promoter        2828..3185
                     /label="SV40 promoter"
                     /note="SV40 enhancer and early promoter"
     CDS             3220..4011
                     /label="NeoR/KanR"
                     /note="aminoglycoside phosphotransferase"
     primer_bind     complement(4202..4221)
                     /label="TK-pA-R"
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    4246..4293
                     /label="HSV TK poly(A) signal"
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      4622..5210
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     promoter        5325..5369
                     /label="CMV"
                     /note="Human cytomegalovirus immediate early
                     enhancer/promoter"
ORIGIN
        1 atatatggag ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa
       61 cgacccccgc ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac
      121 tttccattga cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca
      181 agtgtatcat atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg
      241 gcattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt
      301 agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg
      361 gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg
      421 gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat
      481 gggcggtagg cgtgtacggt gggaggtcta tataagcaga gctggtttag tgaaccgtca
      541 gatccgctag cgctaccggt cgccaccatg gcctcctccg agaacgtcat caccgagttc
      601 atgcgcttca aggtgcgcat ggagggcacc gtgaacggcc acgagttcga gatcgagggc
      661 gagggcgagg gccgccccta cgagggccac aacaccgtga agctgaaggt gaccaagggc
      721 ggccccctgc ccttcgcctg ggacatcctg tccccccagt tccagtacgg ctccaaggtg
      781 tacgtgaagc accccgccga catccccgac tacaagaagc tgtccttccc cgagggcttc
      841 aagtgggagc gcgtgatgaa cttcgaggac ggcggcgtgg cgaccgtgac ccaggactcc
      901 tccctgcagg acggctgctt catctacaag gtgaagttca tcggcgtgaa cttcccctcc
      961 gacggccccg tgatgcagaa gaagaccatg ggctgggagg cctccaccga gcgcctgtac
     1021 ccccgcgacg gcgtgctgaa gggcgagacc cacaaggccc tgaagctgaa ggacggcggc
     1081 cactacctgg tggagttcaa gtccatctac atggccaaga agcccgtgca gctgcccggc
     1141 tactactacg tggacgccaa gctggacatc acctcccaca acgaggacta caccatcgtg
     1201 gagcagtacg agcgcaccga gggccgccac cacctgttcc tgagatctgc cgccatggca
     1261 tatccatatg acgtcccaga ctatgctggc gctagtcgag gcgcaacaag acccaacggg
     1321 ccaaatactg gaaataaaat atgccagttc aaactagtac ttctgggaga gtctgctgtt
     1381 ggcaaatcaa gcctagtgct tcgttttgtg aagggccaat ttcatgaatt tcaagagagt
     1441 accatagggg ctgcttttct aacccaaact gtgtgtcttg atgatacaac agtaaagttt
     1501 gaaatatggg atacagctgg tcaagaacga taccatagct tagcaccaat gtactacaga
     1561 ggagcacaag cagccatagt tgtatatgat atcacaaatg aggagtcctt tgccagagcc
     1621 aaaaactggg ttaaagaact tcagaggcaa gccagtccta acattgtaat agctttatca
     1681 ggaaacaagg ctgatcttgc aaataaaaga gctgtcgatt tccaggaagc acagtcctat
     1741 gcagatgaca acagtttatt attcatggag acatcagcta aaacatcgat gaatgtaaat
     1801 gaaatattca tggcaatagc taaaaagttg ccaaagaacg aaccacagaa tccaggagca
     1861 aattctgcca gaggaagagg agtagacctt actgaaccca cgcagccaac caggaatcag
     1921 tgttgtagta actagctcga gctcaagctt cgaattctgc agtcgacggt accgcgggcc
     1981 cgggatccac cggatctaga taactgatca taatcagcca taccacattt gtagaggttt
     2041 tacttgcttt aaaaaacctc ccacacctcc ccctgaacct gaaacataaa atgaatgcaa
     2101 ttgttgttgt taacttgttt attgcagctt ataatggtta caaataaagc aatagcatca
     2161 caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca
     2221 tcaatgtatc ttaacgcgta aattgtaagc gttaatattt tgttaaaatt cgcgttaaat
     2281 ttttgttaaa tcagctcatt ttttaaccaa taggccgaaa tcggcaaaat cccttataaa
     2341 tcaaaagaat agaccgagat agggttgagt gttgttccag tttggaacaa gagtccacta
     2401 ttaaagaacg tggactccaa cgtcaaaggg cgaaaaaccg tctatcaggg cgatggccca
     2461 ctacgtgaac catcacccta atcaagtttt ttggggtcga ggtgccgtaa agcactaaat
     2521 cggaacccta aagggagccc ccgatttaga gcttgacggg gaaagccggc gaacgtggcg
     2581 agaaaggaag ggaagaaagc gaaaggagcg ggcgctaggg cgctggcaag tgtagcggtc
     2641 acgctgcgcg taaccaccac acccgccgcg cttaatgcgc cgctacaggg cgcgtcaggt
     2701 ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca
     2761 aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg
     2821 aagagtcctg aggcggaaag aaccagctgt ggaatgtgtg tcagttaggg tgtggaaagt
     2881 ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca
     2941 ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt
     3001 agtcagcaac catagtcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt
     3061 ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg
     3121 cctcggcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc ctaggctttt
     3181 gcaaagatcg atcaagagac aggatgagga tcgtttcgca tgattgaaca agatggattg
     3241 cacgcaggtt ctccggccgc ttgggtggag aggctattcg gctatgactg ggcacaacag
     3301 acaatcggct gctctgatgc cgccgtgttc cggctgtcag cgcaggggcg cccggttctt
     3361 tttgtcaaga ccgacctgtc cggtgccctg aatgaactgc aagacgaggc agcgcggcta
     3421 tcgtggctgg ccacgacggg cgttccttgc gcagctgtgc tcgacgttgt cactgaagcg
     3481 ggaagggact ggctgctatt gggcgaagtg ccggggcagg atctcctgtc atctcacctt
     3541 gctcctgccg agaaagtatc catcatggct gatgcaatgc ggcggctgca tacgcttgat
     3601 ccggctacct gcccattcga ccaccaagcg aaacatcgca tcgagcgagc acgtactcgg
     3661 atggaagccg gtcttgtcga tcaggatgat ctggacgaag agcatcaggg gctcgcgcca
     3721 gccgaactgt tcgccaggct caaggcgagc atgcccgacg gcgaggatct cgtcgtgacc
     3781 catggcgatg cctgcttgcc gaatatcatg gtggaaaatg gccgcttttc tggattcatc
     3841 gactgtggcc ggctgggtgt ggcggaccgc tatcaggaca tagcgttggc tacccgtgat
     3901 attgctgaag agcttggcgg cgaatgggct gaccgcttcc tcgtgcttta cggtatcgcc
     3961 gctcccgatt cgcagcgcat cgccttctat cgccttcttg acgagttctt ctgagcggga
     4021 ctctggggtt cgaaatgacc gaccaagcga cgcccaacct gccatcacga gatttcgatt
     4081 ccaccgccgc cttctatgaa aggttgggct tcggaatcgt tttccgggac gccggctgga
     4141 tgatcctcca gcgcggggat ctcatgctgg agttcttcgc ccaccctagg gggaggctaa
     4201 ctgaaacacg gaaggagaca ataccggaag gaacccgcgc tatgacggca ataaaaagac
     4261 agaataaaac gcacggtgtt gggtcgtttg ttcataaacg cggggttcgg tcccagggct
     4321 ggcactctgt cgatacccca ccgagacccc attggggcca atacgcccgc gtttcttcct
     4381 tttccccacc ccacccccca agttcgggtg aaggcccagg gctcgcagcc aacgtcgggg
     4441 cggcaggccc tgccatagcc tcaggttact catatatact ttagattgat ttaaaacttc
     4501 atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc
     4561 cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt
     4621 cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac
     4681 cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct
     4741 tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact
     4801 tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg
     4861 ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata
     4921 aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga
     4981 cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag
     5041 ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg
     5101 agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac
     5161 ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca
     5221 acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg
     5281 cgttatcccc tgattctgtg gataaccgta ttaccgccat gcattagtta ttaatagtaa
     5341 tcaattacgg ggtcattagt tcatagccc
//
