LOCUS       40924_295        7129 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Control vector ARR6-LUC-NOS-At5g62920, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7129)
  AUTHORS   Hwang I, Sheen J.
  TITLE     Two-component circuitry in Arabidopsis cytokinin signal transduction
  JOURNAL   Nature 413 (6854), 383-389 (2001)
  PUBMED    11574878
REFERENCE   2  (bases 1 to 7129)
  AUTHORS   Sheen J.
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2006) Molecular Biology, Massachusetts General 
            Hospital, 185 Cambridge St. Simches Research Building-CPZN7600, 
            Boston, MA 02114, USA
REFERENCE   3  (bases 1 to 7129)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 7129)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature"; 
            date: "2001"; volume: "413"; issue: "6854"; pages: "383-389"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (27-OCT-2006) Molecular Biology, Massachusetts General Hospital, 185
            Cambridge St. Simches Research Building-CPZN7600, Boston, MA 02114, 
            USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..7129
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(15..31)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(39..55)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(63..93)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(108..129)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(417..1005)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(1179..2036)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(2037..2141)
                     /label=AmpR promoter
     primer_bind     2615..2631
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     regulatory      2667..5065
                     /label=ARR6 promoter
                     /note="ARR6 promoter"
                     /regulatory_class="promoter"
     regulatory      4996..5005
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             5066..6715
                     /label=luciferase
                     /note="firefly luciferase"
     terminator      6873..7124
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
ORIGIN
        1 aattcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca
       61 cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa
      121 ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag
      181 ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc
      241 gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct
      301 cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg
      361 tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc
      421 cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga
      481 aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct
      541 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg
      601 gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag
      661 ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat
      721 cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac
      781 aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac
      841 tacggctaca ctagaaggac agtatttggt atctgcgctc tgctgaagcc agttaccttc
      901 ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt
      961 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc
     1021 ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg
     1081 agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca
     1141 atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca
     1201 cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag
     1261 ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac
     1321 ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc
     1381 agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct
     1441 agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc
     1501 gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg
     1561 cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc
     1621 gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat
     1681 tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag
     1741 tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat
     1801 aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg
     1861 cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca
     1921 cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga
     1981 aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc
     2041 ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag cggatacata
     2101 tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg
     2161 ccacctgacg tctaagaaac cattattatc atgacattaa cctataaaaa taggcgtatc
     2221 acgaggccct ttcgtctcgc gcgtttcggt gatgacggtg aaaacctctg acacatgcag
     2281 ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag
     2341 ggcgcgtcag cgggtgttgg cgggtgtcgg ggctggctta actatgcggc atcagagcag
     2401 attgtactga gagtgcacca tatgcggtgt gaaataccgc acagatgcgt aaggagaaaa
     2461 taccgcatca ggcgccattc gccattcagg ctgcgcaact gttgggaagg gcgatcggtg
     2521 cgggcctctt cgctattacg ccagctggcg aaagggggat gtgctgcaag gcgattaagt
     2581 tgggtaacgc cagggttttc ccagtcacga cgttgtaaaa cgacggccag tgccaagctt
     2641 gcatgcctgc aggctctaga ggatccagct agtgcaggta cataagtcaa aagtcacaac
     2701 atatagtaag tacatcataa gttcatttct cttgaaaacg atctctagtg atgatgcact
     2761 ttttaggtat ttattcacat ccgagagttt tctaaatcac aacattgaaa cgtgttcatg
     2821 aatttaaatc aaaacaaacc tgctcatgaa tttggggatg atcgttataa gcaacgctct
     2881 tcaaaagctt tcgatgggtt tttaccatcg atatgcgaat gatgggggaa atcaaacggc
     2941 atttcttcgg gagagagcca agcttctcta aaaactctcg agacttcatc aaaaactacc
     3001 ccattgcctt caggcgtgag atggagccca tcactgctta tagccaacaa caacgagcaa
     3061 gaaccattta taaacaacaa acatgattga ggaggagatg agtgtttgtg aaaaaccaac
     3121 aacatttcag atcagactta gatacagacc ttaggtactt tttctgccaa tcattggttt
     3181 cctgcatctt agaccataag ttgacacatc gcagaccgag ttcctcggcc aatgcaacac
     3241 aatgttgtgc atatacccct gttgtttcgt ttgttctctc aggctctttc atagctttct
     3301 caccgtagat tgatctgatg taacrcaatc actggctcat ttaagaaaca tgtcggacta
     3361 aatgctttga gaaacaaaaa tgcaaagaag aaaaggatca taataaagcc tactctgcat
     3421 aactttgacg tccagcttca tcaattggtg gtggagttat aagcacaatt agcattgtag
     3481 gtgaacattt ctacaaaaca gagtcattag tctttaaagg aatcacactt caagaaaagt
     3541 aagtccatgc attagagtgg atcaaagaag catatcaaaa ccttcaaatg ctgaacaatc
     3601 tttctgacat tatctgtgta ctcttccacc ggcacatgtt gtctatcact ggttcttcct
     3661 ttgagagctg catcgtttgc accgaagaat atcgtcgtag caacaggagg agacgaagag
     3721 ccctaggcaa aaagacagag tagaacacat actgagatag gcacaccaaa acaacacgca
     3781 ctacaagaag agttctcatg atatatgcag atgtaattgc cttttaccat ctcaaaattc
     3841 aaatctaaat ctttcgtact tcaaccaatc catgacaatg tcataaaacc aacacatcct
     3901 agaatcatat caaaaccatt cagaactgaa ccataacaag cataagcaaa acaaaaaatg
     3961 atgcacaaga gacaataact caagatctat atccacacag atcatacaca caatcacaac
     4021 agctcatgaa caaaatcatc ccaatgacta ccaaaattcg ttcgtgttta gatcaaattt
     4081 caaatcaaca tttgtagact cgagggaggg agagagagat aaaaagtact gacgagaggg
     4141 aagatgtgat gaagcaagaa gagagcccat cgggtgttgt agccgccgta gcctcgaacc
     4201 acaacatcag ccttgcgaga gtaagcgtcg gcaagagcag atccccaacc gccggaccta
     4261 aaagactgcg ccgtgatcga gtcgccgaac agaactatct ccggcctcat ctcgctgtct
     4321 ccctccggtt cactgagtaa accgttaact gtattaggaa acttaaagtc tcttctgggc
     4381 caaatcatat gggccgggcc tttagtatta atttgttata tttttggtga atgaaaaggt
     4441 ggtaatctaa agaggtttta ctaattgtag attaaggatg cgtgtaatgt tttagtatta
     4501 gtttatgatt tcttgatttc acatttttct gtcttgagct taagtcaaaa cctcaaagtg
     4561 aacttctacg tagatgaagt attgaagtgt agaagttaaa tgcgtgaact tccacaattc
     4621 aactacaaac acacttttac caaaaaaaac tacaaacaca cacaaataat ttaaaaataa
     4681 aaaaagaatt ctacaagtta ttgaatatcg gtttgggtcg gttaaatctt gcatcccatt
     4741 ccaaattttc tggtttgttc ttcgttttga ctaaacttga accgattggt ttgactttat
     4801 ttcggttttt tgttttaact tatttaataa taaaaatcca ccgaactatt atttatataa
     4861 aatataaaat aaagattttg aaagcaaatt gacaaaatct taaagatatg caaaatctct
     4921 cgatttcatt catatttttt ccctctttta tttctttctt tctttctttc tacttatata
     4981 aatggcccaa ccactgccac catggtttca catcatatct ttctcttcct ttttcttcca
     5041 aatccatcaa cattcgttga tcaccatgga agacgccaaa aacataaaga aaggcccggc
     5101 gccattctat cctctagagg atggaaccgc tggagagcaa ctgcataagg ctatgaagag
     5161 atacgccctg gttcctggaa caattgcttt tacagatgca catatcgagg tgaacatcac
     5221 gtacgcggaa tacttcgaaa tgtccgttcg gttggcagaa gctatgaaac gatatgggct
     5281 gaatacaaat cacagaatcg tcgtatgcag tgaaaactct cttcaattct ttatgccggt
     5341 gttgggcgcg ttatttatcg gagttgcagt tgcgcccgcg aacgacattt ataatgaacg
     5401 tgaattgctc aacagtatga acatttcgca gcctaccgta gtgtttgttt ccaaaaaggg
     5461 gttgcaaaaa attttgaacg tgcaaaaaaa attaccaata atccagaaaa ttattatcat
     5521 ggattctaaa acggattacc agggatttca gtcgatgtac acgttcgtca catctcatct
     5581 acctcccggt tttaatgaat acgattttgt accagagtcc tttgatcgtg acaaaacaat
     5641 tgcactgata atgaattcct ctggatctac tgggttacct aagggtgtgg cccttccgca
     5701 tagaactgcc tgcgtcagat tctcgcatgc cagagatcct atttttggca atcaaatcat
     5761 tccggatact gcgattttaa gtgttgttcc attccatcac ggttttggaa tgtttactac
     5821 actcggatat ttgatatgtg gatttcgagt cgtcttaatg tatagatttg aagaagagct
     5881 gtttttacga tcccttcagg attacaaaat tcaaagtgcg ttgctagtac caaccctatt
     5941 ttcattcttc gccaaaagca ctctgattga caaatacgat ttatctaatt tacacgaaat
     6001 tgcttctggg ggcgcacctc tttcgaaaga agtcggggaa gcggttgcaa aacgcttcca
     6061 tcttccaggg atacgacaag gatatgggct cactgagact acatcagcta ttctgattac
     6121 acccgagggg gatgataaac cgggcgcggt cggtaaagtt gttccatttt ttgaagcgaa
     6181 ggttgtggat ctggataccg ggaaaacgct gggcgttaat cagagaggcg aattatgtgt
     6241 cagaggacct atgattatgt ccggttatgt aaacaatccg gaagcgacca acgccttgat
     6301 tgacaaggat ggatggctac attctggaga catagcttac tgggacgaag acgaacactt
     6361 cttcatagtt gaccgcttga agtctttaat taaatacaaa ggatatcagg tggcccccgc
     6421 tgaattggaa tcgatattgt tacaacaccc caacatcttc gacgcgggcg tggcaggtct
     6481 tcccgacgat gacgccggtg aacttcccgc cgccgttgtt gttttggagc acggaaagac
     6541 gatgacggaa aaagagatcg tggattacgt cgccagtcaa gtaacaaccg cgaaaaagtt
     6601 gcgcggagga gttgtgtttg tggacgaagt accgaaaggt cttaccggaa aactcgacgc
     6661 aagaaaaatc agagagatcc tcataaaggc caagaagggc ggaaagtcca aattgtaaaa
     6721 tgtaactgta ttcagcgatg acgaaattct tagctattgt aatattatat gcaaattgat
     6781 gaatggtaat tttgtaattg tgggtcactg tactatttta acgaataata aaatcaggta
     6841 taggtaacta aaaaggaatt cgagctcgaa ttgatcgttc aaacatttgg caataaagtt
     6901 tcttaagatt gaatcctgtt gccggtcttg cgatgattat catataattt ctgttgatta
     6961 cgttaagcat gtaataatta acatgtaatg catgacgtta tttatgagat gggtttttat
     7021 gattagagtc ccgcaattat acatttaata cgcgatagaa aacaaaatat agcgcgcaaa
     7081 ctaggataaa ttatcgcgcg cggtgtcatc tatgttacta gatctctag
//
