LOCUS       .                       5896 bp    DNA     circular UNK 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   
VERSION     
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     repeat_region   complement(1..141)
                     /label="AAV2 ITR"
                     /note="inverted terminal repeat of adeno-associated virus
                     serotype 2"
     protein_bind    148..168
                     /label="attB4"
                     /note="core recombination site for the Gateway® BP
                     reaction"
     polyA_signal    238..286
                     /label="poly(A) signal"
                     /note="synthetic polyadenylation signal"
     misc_feature    300..391
                     /label="pause site"
                     /note="RNA polymerase II transcriptional pause signal from
                     the human α2 globin gene"
     protein_bind    1137..1261
                     /label="attR1"
                     /note="recombination site for the Gateway® LR reaction"
     promoter        1298..1328
                     /label="lac UV5 promoter"
                     /note="E. coli lac promoter with an 'up' mutation"
     CDS             1382..2038
                     /label="CmR"
                     /note="chloramphenicol acetyltransferase"
     CDS             2383..2685
                     /label="ccdB"
                     /note="CcdB, a bacterial toxin that poisons DNA gyrase"
     protein_bind    complement(2729..2853)
                     /label="attR2"
                     /note="recombination site for the Gateway® LR reaction"
     polyA_signal    complement(2911..3032)
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     repeat_region   3159..3299
                     /label="AAV2 ITR"
                     /note="inverted terminal repeat of adeno-associated virus
                     serotype 2"
     rep_origin      3374..3829
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        4111..4215
                     /label="AmpR promoter"
     CDS             4216..5073
                     /label="AmpR"
                     /note="β-lactamase"
     rep_origin      5247..5835
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc
       61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca
      121 actccatcac taggggttcc ttctagacaa ctttgtatag aaaagttggg taccactagt
      181 acgtctctca aggataagta agtaatatta aggtacggga ggtattggac aggccgcaat
      241 aaaatatctt tattttcatt acatctgtgt gttggttttt tgtgtgaatc gatagtacta
      301 acatacgctc tccatcaaaa caaaacgaaa caaaacaaac tagcaaaata ggctgtcccc
      361 agtgcaagtg caggtgccag aacatttctc tgacctaact ggccggtacc tgagctcctc
      421 actgactccg tcctggagtt ggatgagaga taatggcctt acgttgtgcc aggggagggt
      481 cgggctggat ttagcaagat ttaccttctc caaagagcgg tgctgcagtg gcacagctgc
      541 ccacggaggt gggggggtca ccgtccctgg aggtgatgaa gaactgtggg gatgtggcac
      601 tgagggacat ggccagtggg cacggtgggt gggttggggt tggtcttggg gatcttggag
      661 ggcttttcca gccttcatga tttgacgatt gtatgaacat ctacatggca attctccagc
      721 tgcctgtccc agtcctactg acccagctgt atctctccag gcaagctctt ccaccccttc
      781 tgcttgcatc cagacaccat caaacatgca ggctcagaca caagcctaaa gctttttccc
      841 cgtatccccc caggtgtctg caggctcaaa gagcagcgag aagcgttcag aggaaagcga
      901 tcccgtgcca ccttccccgt gcccgggctg tccccgcacg ctgccggctc ggggatgcgg
      961 ggggagcgcc ggaccggagc ggagccccgg gcggctcgct gctgccccct agcgggggag
     1021 ggacgtaatt acatccctgg gggctttggg ggggggctgt ccccgtcgtg caggtgccag
     1081 aacatttctc tggcctaact ggccggtacc agtgtggtgg aattctgcag atatcaacaa
     1141 gtttgtacaa aaaagctgaa cgagaaacgt aaaatgatat aaatatcaat atattaaatt
     1201 agattttgca taaaaaacag actacataat actgtaaaac acaacatatc cagtcactat
     1261 ggcggcccct cgaggggccg cattaggcac cccaggcttt acactttatg cttccggctc
     1321 gtataatgtg tggattttga gttaggatcc gtcgagattt tcaggagcta aggaagctaa
     1381 aatggagaaa aaaatcactg gatataccac cgttgatata tcccaatggc atcgtaaaga
     1441 acattttgag gcatttcagt cagttgctca atgtacctat aaccagaccg ttcagctgga
     1501 tattacggcc tttttaaaga ccgtaaagaa aaataagcac aagttttatc cggcctttat
     1561 tcacattctt gcccgcctga tgaatgctca tccggaattc cgtatggcaa tgaaagacgg
     1621 tgagctggtg atatgggata gtgttcaccc ttgttacacc gttttccatg agcaaactga
     1681 aacgttttca tcgctctgga gtgaatacca cgacgatttc cggcagtttc tacacatata
     1741 ttcgcaagat gtggcgtgtt acggtgaaaa cctggcctat ttccctaaag ggtttattga
     1801 gaatatgttt ttcgtctcag ccaatccctg ggtgagtttc accagttttg atttaaacgt
     1861 ggccaatatg gacaacttct tcgcccccgt tttcaccatg ggcaaatatt atacgcaagg
     1921 cgacaaggtg ctgatgccgc tggcgattca ggttcatcat gccgtttgtg atggcttcca
     1981 tgtcggcaga atgcttaatg aattacaaca gtactgcgat gagtggcagg gcggggcgta
     2041 aagatctgga tccggcttac taaaagccag ataacagtat gcgtatttgc gcgctgattt
     2101 ttgcggtata agaatatata ctgatatgta tacccgaagt atgtcaaaaa gaggtatgct
     2161 atgaagcagc gtattacagt gacagttgac agcgacagct atcagttgct caaggcatat
     2221 atgatgtcaa tatctccggt ctggtaagca caaccatgca gaatgaagcc cgtcgtctgc
     2281 gtgccgaacg ctggaaagcg gaaaatcagg aagggatggc tgaggtcgcc cggtttattg
     2341 aaatgaacgg ctcttttgct gacgagaaca ggggctggtg aaatgcagtt taaggtttac
     2401 acctataaaa gagagagccg ttatcgtctg tttgtggatg tacagagtga tattattgac
     2461 acgcccgggc gacggatggt gatccccctg gccagtgcac gtctgctgtc agataaagtc
     2521 tcccgtgaac tttacccggt ggtgcatatc ggggatgaaa gctggcgcat gatgaccacc
     2581 gatatggcca gtgtgccggt ctccgttatc ggggaagaag tggctgatct cagccaccgc
     2641 gaaaatgaca tcaaaaacgc cattaacctg atgttctggg gaatataaat gtcaggctcc
     2701 cttatacaca gccagtctgc aggtcgacca tagtgactgg atatgttgtg ttttacagta
     2761 ttatgtagtc tgttttttat gcaaaatcta atttaatata ttgatattta tatcatttta
     2821 cgtttctcgt tcagctttct tgtacaaagt ggttggcctc ggcggccaag ctctagagtc
     2881 ggggcggccg gccgcttcga gcagacatga taagatacat tgatgagttt ggacaaacca
     2941 caactagaat gcagtgaaaa aaatgcttta tttgtgaaat ttgtgatgct attgctttat
     3001 ttgtaaccat tataagctgc aataaacaag ttaacaacaa caattgcatt cattttatgt
     3061 ttcaggttca gggggaggtg tgggaggttt tttaaagcaa gtaaaacctc tacaaatgtg
     3121 gtaaaatcga taaggatccg catgctgggg agggccgcag gaacccctag tgatggagtt
     3181 ggccactccc tctctgcgcg ctcgctcgct cactgaggcc gggcgaccaa aggtcgcccg
     3241 acgcccgggc tttgcccggg cggcctcagt gagcgagcga gcgcgcagct gcctgcaggg
     3301 gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatacgtca
     3361 aagcaaccat agtacgcgcc ctgtagcggc gcattaagcg cggcgggggt ggtggttacg
     3421 cgcagcgtga ccgctacact tgccagcgcc ttagcgcccg ctcctttcgc tttcttccct
     3481 tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta
     3541 gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgattt gggtgatggt
     3601 tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg
     3661 ttctttaata gtggactctt gttccaaact ggaacaacac tcaactctat ctcgggctat
     3721 tcttttgatt tataagggat tttgccgatt tcggtctatt ggttaaaaaa tgagctgatt
     3781 taacaaaaat ttaacgcgaa ttttaacaaa atattaacgt ttacaatttt atggtgcact
     3841 ctcagtacaa tctgctctga tgccgcatag ttaagccagc cccgacaccc gccaacaccc
     3901 gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc
     3961 gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgagacga
     4021 aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag
     4081 acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa
     4141 atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat
     4201 tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg
     4261 gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa
     4321 gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt
     4381 gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt
     4441 ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat
     4501 tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg
     4561 acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta
     4621 cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat
     4681 catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag
     4741 cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa
     4801 ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca
     4861 ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc
     4921 ggtgagcgtg gaagccgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt
     4981 atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc
     5041 gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat
     5101 atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt
     5161 tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac
     5221 cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc
     5281 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca
     5341 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta
     5401 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct
     5461 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg
     5521 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc
     5581 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta
     5641 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg
     5701 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt
     5761 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg
     5821 cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg
     5881 ccttttgctc acatgt
//
