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Gene name
WD repeat domain 77
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Organism
Human
- Entrez Gene ID
-
Gene Symbol
WDR77
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Gene Type
protein coding
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Also Known as
p44; MEP50; MEP-50; HKMT1069; Nbla10071; p44/Mep50
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Encoded Protein Alias
methylosome protein 50; WD repeat-containing protein 77; androgen receptor cofactor p44
Accession NO.: NM_024102
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Product Name
WDR77(NM_024102) Human cDNA/ORF Clone
- Accession NO.
-
Transcript Name
Homo sapiens WD repeat domain 77 (WDR77), transcript variant 2, mRNA.
-
Synonyms
-
ORF length
1029
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Annotation
This variant (2) uses an alternate in-frame splice junction in the 3' end compared to variant 1. The resulting isoform (2) has the same N- and C-termini but is shorter compared to isoform 1.
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siRNA
UCAGCAAAGUGAAGUCUUUtt
AGACACUUAUUGUCAGCAAtt
GUGCUGGACUCAAGCCUUUtt
- DataSheet:
- FAQs:
- DataSheet:
- FAQs:
- DataSheet:
- FAQs:
Accession NO.: NM_001317062
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Product Name
WDR77(NM_001317062) Human cDNA/ORF Clone
- Accession NO.
-
Transcript Name
Homo sapiens WD repeat domain 77 (WDR77), transcript variant 1, mRNA.
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Synonyms
-
ORF length
1065
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Annotation
This variant (1) represents the longest transcript and encodes the longest isoform (1).
-
siRNA
UCAGCAAAGUGAAGUCUUUtt
AGACACUUAUUGUCAGCAAtt
GUGCUGGACUCAAGCCUUUtt
- DataSheet:
- FAQs:
Accession NO.: NM_001317064
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Product Name
WDR77(NM_001317064) Human cDNA/ORF Clone
- Accession NO.
-
Transcript Name
Homo sapiens WD repeat domain 77 (WDR77), transcript variant 4, mRNA.
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Synonyms
-
ORF length
837
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Annotation
This variant (4) lacks an alternate in-frame segment in the 5' end and uses an alternate in-frame splice junction in the 3' end compared to variant 1. The resulting isoform (4) has the same N- and C-termini but is shorter compared to isoform 1.
-
siRNA
UCAGCAAAGUGAAGUCUUUtt
GUGCUGGACUCAAGCCUUUtt
- DataSheet:
- FAQs:
Accession NO.: NM_001317063
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Product Name
WDR77(NM_001317063) Human cDNA/ORF Clone
- Accession NO.
-
Transcript Name
Homo sapiens WD repeat domain 77 (WDR77), transcript variant 3, mRNA.
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Synonyms
-
ORF length
1023
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Annotation
This variant (3) uses two alternate in-frame splice junctions in the 3' end compared to variant 1. The resulting isoform (3) has the same N- and C-termini but is shorter compared to isoform 1.
-
siRNA
UCAGCAAAGUGAAGUCUUUtt
AGACACUUAUUGUCAGCAAtt
GUGCUGGACUCAAGCCUUUtt
- DataSheet:
- FAQs:
-
Vector Name
pCMV-SPORT6
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primers
T7: TAATACGACTCACTATAGGG
M13 rev: CAGGAAACAGCTATGAC -
Vector Length
4396
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Vector Type
Mammalian Expression Vector
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Promoter
CMV
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Antibiotic Resistance
Ampicillin
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Selection Marker
-
-
Protein Tag
None
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Description
Vector for cloning and transient mammalian cell expression of cDNAs.
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Vector Name
pOTENT-1
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primers
pOT-F: TAATAGTAATCAATTACGGG
pOT-R: CCTCTACAAATGTGGTATGGC -
Vector Length
7510
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Vector Type
Mammalian Expression Vector
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Promoter
CMV
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Antibiotic Resistance
Kanamycin
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Selection Marker
Neomycin
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Protein Tag
FLAG, 6 His
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Description
ORFs cloned in this vector will be expressed in mammalian cells as a tagged protein with the C-terminal FLAG-6 His tags.
Such clones are the best for detection and purification of the transgene using anti-FLAG or anti-His antibodies.
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Vector Name
pUC19
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primers
M13 fwd: GTAAAACGACGGCCAGT
M13 rev: CAGGAAACAGCTATGAC -
Vector Length
2686
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Vector Type
Cloning Vector
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Promoter
lac
-
Antibiotic Resistance
Ampicillin
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Selection Marker
-
-
Protein Tag
None
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Description
pUC19 is a small, high-copy number E. coli plasmid cloning vector, of which multiple cloning sites as shown below. The molecule is a small double-stranded circle, 2686 base pairs in length. pUC19 encodes the N-terminal fragment of b-galactosidase (lacZa), which allows for blue/white colony screening (i.e., a-complementation), as well as a pUC origin of replication.
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Vector Name
pcDNA3.1-3xFlag
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primers
T7: TAATACGACTCACTATAGGG
BGH: TAGAAGGCACAGTCGAGG -
Vector Length
5508
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Vector Type
Mammalian Expression Vector
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Promoter
CMV
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Antibiotic Resistance
Ampicillin
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Selection Marker
Neomycin
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Protein Tag
Flag
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Description
Mammalian vector for expressing C-terminal 3xFlag tagged proteins.
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