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Gene name
ubiquitin carboxy-terminal hydrolase L1
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Organism
Mouse
- Entrez Gene ID
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Gene Symbol
Uchl1
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Gene Type
protein coding
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Also Known as
gad; C88048; PGP9.5; R75593; UCH-L1; UCHL-1; PGP 9.5; AW822034
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Encoded Protein Alias
ubiquitin carboxyl-terminal hydrolase isozyme L1; gracile axonal dystrophy; neuron cytoplasmic protein 9.5; protein gene product 9.5; ubiquitin thioesterase L1
Accession NO.: NM_011670
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Product Name
Uchl1(NM_011670) Mouse cDNA/ORF Clone
- Accession NO.
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Transcript Name
Mus musculus ubiquitin carboxy-terminal hydrolase L1 (Uchl1), mRNA.
- Synonyms
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ORF length
672
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siRNA
GCCCUAAAGUUUACUUCAUtt
GAUCCGUCCUGAAACAGUUtt
CAUUUUAUUCUGUUCAACAtt
- DataSheet:
- DataSheet:
- DataSheet:
- DataSheet:
- DataSheet:
- FAQs:
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Vector Name
pCMV-SPORT6
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primers
T7: TAATACGACTCACTATAGGG
M13 rev: CAGGAAACAGCTATGAC -
Vector Length
4396
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Vector Type
Mammalian Expression Vector
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Promoter
CMV
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Antibiotic Resistance
Ampicillin
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Selection Marker
-
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Protein Tag
None
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Description
Vector for cloning and transient mammalian cell expression of cDNAs.
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Vector Name
pEXP-Entry
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primers
T7: TAATACGACTCACTATAGG
M13 rev: CAGGAAACAGCTATGAC -
Vector Length
4919
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Vector Type
Mammalian Expression Vector
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Promoter
CMV
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Antibiotic Resistance
Kanamycin
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Selection Marker
Neomycin
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Protein Tag
Myc,FLAG
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Description
ORFs cloned in this vector will be expressed in mammalian cellsas a tagged protein with the C-terminal Myc-FLAG tags.
Such clones are the best for detection and purification of the transgene using anti-Myc or anti-FLAG antibodies.
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Vector Name
pGEM-T
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primers
T7: TAATACGACTCACTATAGGG
M13 rev: CAGGAAACAGCTATGAC -
Vector Length
3000
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Vector Type
Cloning Vector
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Promoter
lac
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Antibiotic Resistance
Ampicillin
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Selection Marker
-
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Protein Tag
None
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Description
The pGEM-T is 3kb in length, and contains the amplicin resistance gene, conferring selection of the plasmid in E. coli, and the ori site which is the bacterial origin of replication. The plasmid has multiple cloning sites as shown below. The coding sequence was inserted by TA cloning. Many E. coli strains are suitable for the propagation of this vector including JM109, DH5α and TOP10.
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