TST Antibody (Center)
Purified Rabbit Polyclonal Antibody (Pab)
IHC-P, WB, ELISA
Thiosulfate sulfurtransferase antibody; Rhodanese antibody; TST antibody
This TST antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 170-203 amino acids from the Central region of human TST.
Short term 4°C, long term aliquot and store at -20°C, avoid freeze thaw cycles. Store undiluted.
IHC-P: 1:25, WB: 1:2000
134841 staining TST in human kidney tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
All lanes : Anti-TST Antibody (Center) at 1:2000 dilution Lane 1: human liver lysate Lane 2: Hela whole cell lysate Lane 3: rat kidney lysate Lane 4: rat liver lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 33 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
Formation of iron-sulfur complexes, cyanide detoxification or modification of sulfur-containing enzymes. Other thiol compounds, besides cyanide, can act as sulfur ion acceptors. Also has weak mercaptopyruvate sulfurtransferase (MST) activity (By similarity). Together with MRPL18, acts as a mitochondrial import factor for the cytosolic 5S rRNA. Only the nascent unfolded cytoplasmic form is able to bind to the 5S rRNA.
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