TSC2 Antibody (Center S1418/S1420)
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
WB, IHC-P, ELISA
TSC4 antibody; Tuberin antibody; Tuberous sclerosis 2 protein antibody; TSC2 antibody; TSC4 antibody
This TSC2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1397-1426 amino acids from the Central region of human TSC2.
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Short term 4°C, long term aliquot and store at -20°C, avoid freeze thaw cycles. Store undiluted.
IHC-P: 1:25, WB: 1:1000
Immunohistochemical analysis of paraffin-embedded H. brain section using TSC2 Antibody (Center S1418/S1420)(Cat#121871). 121871 was diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.
TSC2 Antibody (Center S1418/S1420) (Cat. #121871) western blot analysis in 293 cell line lysates (35ug/lane).This demonstrates the TSC2 antibody detected the TSC2 protein (arrow).
In complex with TSC1, inhibits the nutrient-mediated or growth factor-stimulated phosphorylation of S6K1 and EIF4EBP1 by negatively regulating mTORC1 signaling. Acts as a GTPase- activating protein (GAP) for the small GTPase RHEB, a direct activator of the protein kinase activity of mTORC1. Implicated as a tumor suppressor. Involved in microtubule-mediated protein transport, but this seems to be due to unregulated mTOR signaling. Stimulates weakly the intrinsic GTPase activity of the Ras-related proteins RAP1A and RAB5 in vitro. Mutations in TSC2 lead to constitutive activation of RAP1A in tumors.
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