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Product Name
SPTAN1 Polyclonal Antibody
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Description
Polyclonal antibody to SPTAN1
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Tested applications
WB, IHC, IF
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Species reactivity
Human, Mouse, Rat
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Alternative names
SPTAN1 antibody; EIEE5 antibody; NEAS antibody; SPTA2 antibody; spectrin alpha, non-erythrocytic 1 antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 950-1130 of human SPTAN1 (NP_001123910.1).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200 -
Validations
Western blot - SPTAN1 Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using SPTAN1 antibody at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 1s.
Immunofluorescence - SPTAN1 Polyclonal Antibody
Immunofluorescence analysis of U-2 OS cells using SPTAN1 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence - SPTAN1 Polyclonal Antibody
Immunofluorescence analysis of U-2 OS cells using SPTAN1 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
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Background
Fodrin, which seems to be involved in secretion, interacts with calmodulin in a calcium-dependent manner and is thus candidate for the calcium-dependent movement of the cytoskeleton at the membrane.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"