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  • SARS-CoV-2 Spike RBD Protein (Fc Tag)

SARS-CoV-2 Spike RBD Protein (Fc Tag)

Cat.#: 521009

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Product Information

  • Product Name
    SARS-CoV-2 Spike RBD Protein (Fc Tag)
  • Documents
  • Description
    The spike (S) glycoprotein of coronaviruses contains protrusions that will only bind to certain receptors on the host cell. Known receptors bind S1 are ACE2, angiotensin-converting enzyme 2; DPP4, dipeptidyl peptidase-4; APN, aminopeptidase N; CEACAM, carcinoembryonic antigen-related cell adhesion molecule 1; Sia, sialic acid; O-ac Sia, O-acetylated sialic acid. The spike is essential for both host specificity and viral infectivity. The term 'peplomer' is typically used to refer to a grouping of heterologous proteins on the virus surface that function together. The spike (S) glycoprotein of coronaviruses is known to be essential in the binding of the virus to the host cell at the advent of the infection process. It's been reported that SARS-CoV-2 (COVID-19 coronavirus, 2019-nCoV) can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity. The main functions for the Spike protein are summarized as: Mediate receptor binding and membrane fusion; Defines the range of the hosts and specificity of the virus; Main component to bind with the neutralizing antibody; Key target for vaccine design; Can be transmitted between different hosts through gene recombination or mutation of the receptor binding domain (RBD), leading to a higher mortality rate.
  • Source/Expression Host
    HEK293
  • Expression Plasmid/cDNA
    A DNA sequence encoding the SARS-CoV-2 (2019-nCoV) Spike Protein (RBD) (YP_009724390.1) (Arg319-Phe541) was expressed with the Fc tag at the C-terminus.
  • Protein Species
    2019-nCoV
  • Molecular weight
    The recombinant SARS-CoV-2 (2019-nCoV) Spike Protein (RBD, Fc Tag) has a predicted molecular mass of 48 kDa. As a result of glycosylation, it migrates as an approximately 65 kDa band in SDS-PAGE under reducing conditions.
  • Purity
    >90%
  • Activity
    Measured by its binding ability in a functional ELISA. Immobilized human ACE2 protein (His tag) at 2μg/mL (100μL/well) can bind SARS-CoV-2 (2019-nCoV) Spike Protein (RBD, Fc Tag), the EC50 of 2019-nCoV Spike Protein (RBD, Fc Tag) is 2-6 ng/mL.
  • Validations

    SARS-CoV-2 Spike RBD Protein (Fc Tag)

    SARS-CoV-2 Spike RBD Protein (Fc Tag)

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