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  • Pkm Mouse cDNA/ORF Clone

Pkm Mouse cDNA/ORF Clone

Cat.#: 717376-1

  • Accession#:
  • Vector:
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Product Information

  • Gene name
    pyruvate kinase, muscle
  • Organism
    Mouse
  • Entrez Gene ID
    18746
  • Gene Symbol
    Pkm
  • Gene Type
    protein coding
  • Also Known as
    Pk3; Pk-2; Pk-3; Pkm2; AA414905; AL024370; AL024424
  • Encoded Protein Alias
    pyruvate kinase 3; pyruvate kinase isozymes M1/M2; pyruvate kinase muscle isozyme
  • Product Name
    Pkm(NM_001253883) Mouse cDNA/ORF Clone
  • Accession NO.
    NM_001253883
  • Transcript Name
    Mus musculus pyruvate kinase, muscle (Pkm), transcript variant 1, mRNA.
  • Synonyms
    XM_011242673  
  • ORF length
    1596
  • Annotation
    This variant (1) encodes isoform M1. Isoforms M1 and M2 are identical in length but have different sequences.
  • siRNA

    GGAUGUCGACCUUCGUGUAtt

    AGAUCAUCAGCAAAAUCGAtt

    ACAUCAAGAUCAUCAGCAAtt

    CCAUCAAGAAUGUCCGUGAtt

  • DataSheet:
    NM_001253883 in pGEM-T
  • FAQs:
    DNA products or services FAQs
  • Vector Name
    pEXP-Entry
  • primers
    T7: TAATACGACTCACTATAGG
    M13 rev: CAGGAAACAGCTATGAC
  • Vector Length
    4919
  • Vector Type
    Mammalian Expression Vector
  • Promoter
    CMV
  • Antibiotic Resistance
    Kanamycin
  • Selection Marker
    Neomycin
  • Protein Tag
    Myc,FLAG
  • Description
    ORFs cloned in this vector will be expressed in mammalian cellsas a tagged protein with the C-terminal Myc-FLAG tags.
    Such clones are the best for detection and purification of the transgene using anti-Myc or anti-FLAG antibodies.
  • Vector Name
    pEnCMV
  • primers
    CMV fwd: cg caaatgggcg gtaggcgtg
    BGH: TAGAAGGCACAGTCGAGG
  • Vector Length
    5936
  • Vector Type
    Mammalian Expression Vector
  • Promoter
    CMV
  • Antibiotic Resistance
    Ampicillin
  • Selection Marker
    Neomycin
  • Protein Tag
    None
  • Description
  • Vector Name
    pGEM-T
  • primers
    T7: TAATACGACTCACTATAGGG
    M13 rev: CAGGAAACAGCTATGAC
  • Vector Length
    3000
  • Vector Type
    Cloning Vector
  • Promoter
    lac
  • Antibiotic Resistance
    Ampicillin
  • Selection Marker
    -
  • Protein Tag
    None
  • Description
    The pGEM-T is 3kb in length, and contains the amplicin resistance gene, conferring selection of the plasmid in E. coli, and the ori site which is the bacterial origin of replication. The plasmid has multiple cloning sites as shown below. The coding sequence was inserted by TA cloning. Many E. coli strains are suitable for the propagation of this vector including JM109, DH5α and TOP10.
  • Vector Name
    pUC19
  • primers
    M13 fwd: GTAAAACGACGGCCAGT
    M13 rev: CAGGAAACAGCTATGAC
  • Vector Length
    2686
  • Vector Type
    Cloning Vector
  • Promoter
    lac
  • Antibiotic Resistance
    Ampicillin
  • Selection Marker
    -
  • Protein Tag
    None
  • Description
    pUC19 is a small, high-copy number E. coli plasmid cloning vector, of which multiple cloning sites as shown below. The molecule is a small double-stranded circle, 2686 base pairs in length. pUC19 encodes the N-terminal fragment of b-galactosidase (lacZa), which allows for blue/white colony screening (i.e., a-complementation), as well as a pUC origin of replication.
  • Vector Name
    pcDNA3.1-3xFlag
  • primers
    T7: TAATACGACTCACTATAGGG
    BGH: TAGAAGGCACAGTCGAGG
  • Vector Length
    5494
  • Vector Type
    Mammalian Expression Vector
  • Promoter
    CMV
  • Antibiotic Resistance
    Ampicillin
  • Selection Marker
    Neomycin
  • Protein Tag
    Flag
  • Description
    Mammalian vector for expressing C-terminal 3xFlag tagged proteins.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"