Puma BH3 Domain Antibody
Purified Rabbit Polyclonal Antibody (Pab)
WB, IHC-P, IF, FC, ELISA
PUMA antibody; Bcl-2-binding component 3 antibody; JFY-1 antibody; p53 up-regulated modulator of apoptosis antibody; BBC3 antibody; PUMA antibody
This Puma BH3 Domain antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 119-154 amino acids from human Puma BH3 Domain.
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Short term 4°C, long term aliquot and store at -20°C, avoid freeze thaw cycles. Store undiluted.
WB: 1:1000, IHC-P: 1:10~50, IF: 1:10~50, FC: 1:10~50
Western blot analysis of anti-Puma BH3 domain Pab (Cat. #134831) in HL-60 cell lysate. Puma BH3 domain (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.
Formalin-fixed and paraffin-embedded human breast carcinoma tissue reacted with Puma BH3 Domain antibody (Cat.#134831), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Confocal immunofluorescent analysis of Puma BH3 Domain Antibody(Cat#134831) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).
Puma BH3 Domain Antibody (Cat. #134831) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Essential mediator of p53/TP53-dependent and p53/TP53- independent apoptosis. Functions by promoting partial unfolding of BCL2L1 and dissociation of BCL2L1 from p53/TP53. Regulates ER stress-induced neuronal apoptosis.
- Vesela B, Matalova E. Detection of Bim and Puma in mouse hair follicles using immunofluorescence and TUNEL assay double staining. Biotechnic & histochemistry : official publication of the Biological Stain Commission. 90(8):587-93. 2015.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"