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Gene name
prohibitin
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Organism
Human
- Entrez Gene ID
-
Gene Symbol
PHB
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Gene Type
protein coding
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Also Known as
PHB1; HEL-215; HEL-S-54e
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Encoded Protein Alias
prohibitin; epididymis luminal protein 215; epididymis secretory sperm binding protein Li 54e
Accession NO.: NM_001281497
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Product Name
PHB(NM_001281497) Human cDNA/ORF Clone
- Accession NO.
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Transcript Name
Homo sapiens prohibitin (PHB), transcript variant 3, mRNA.
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Synonyms
-
ORF length
468
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Annotation
This variant (3) uses alternate in-frame splice sites and lacks an alternate in-frame exon in the coding region compared to variant 1. It encodes isoform 2 which is shorter compared to isoform 1.
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siRNA
CGUGGGUACAGAAACCAAUtt
GCAUCGGAGAGGACUAUGAtt
UCACAACUGAGAUCCUCAAtt
- DataSheet:
- FAQs:
Accession NO.: XM_017024762
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Product Name
PHB(XM_017024762) Human cDNA/ORF Clone
- Accession NO.
-
Transcript Name
PREDICTED: Homo sapiens prohibitin (PHB), transcript variant X1, mRNA.
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Synonyms
-
ORF length
870
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siRNA
CGUGGGUACAGAAACCAAUtt
GCAUCGGAGAGGACUAUGAtt
UCACAACUGAGAUCCUCAAtt
- DataSheet:
- FAQs:
Accession NO.: NM_002634
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Product Name
PHB(NM_002634) Human cDNA/ORF Clone
- Accession NO.
-
Transcript Name
Homo sapiens prohibitin (PHB), transcript variant 1, mRNA.
- Synonyms
-
ORF length
819
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Annotation
This variant (1) encodes isoform 1. Variants 1, 2 and 4 encode the same isoform (1).
-
siRNA
CGUGGGUACAGAAACCAAUtt
GCAUCGGAGAGGACUAUGAtt
UCACAACUGAGAUCCUCAAtt
- DataSheet:
- DataSheet:
- DataSheet:
- FAQs:
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Vector Name
pEXP-Entry
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primers
T7: TAATACGACTCACTATAGG
M13 rev: CAGGAAACAGCTATGAC -
Vector Length
4919
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Vector Type
Mammalian Expression Vector
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Promoter
CMV
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Antibiotic Resistance
Kanamycin
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Selection Marker
Neomycin
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Protein Tag
Myc,FLAG
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Description
ORFs cloned in this vector will be expressed in mammalian cellsas a tagged protein with the C-terminal Myc-FLAG tags.
Such clones are the best for detection and purification of the transgene using anti-Myc or anti-FLAG antibodies.
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Vector Name
pGEM-T
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primers
T7: TAATACGACTCACTATAGGG
M13 rev: CAGGAAACAGCTATGAC -
Vector Length
3000
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Vector Type
Cloning Vector
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Promoter
lac
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Antibiotic Resistance
Ampicillin
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Selection Marker
-
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Protein Tag
None
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Description
The pGEM-T is 3kb in length, and contains the amplicin resistance gene, conferring selection of the plasmid in E. coli, and the ori site which is the bacterial origin of replication. The plasmid has multiple cloning sites as shown below. The coding sequence was inserted by TA cloning. Many E. coli strains are suitable for the propagation of this vector including JM109, DH5α and TOP10.
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Vector Name
pOTENT-1
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primers
pOT-F: TAATAGTAATCAATTACGGG
pOT-R: CCTCTACAAATGTGGTATGGC -
Vector Length
7510
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Vector Type
Mammalian Expression Vector
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Promoter
CMV
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Antibiotic Resistance
Kanamycin
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Selection Marker
Neomycin
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Protein Tag
FLAG, 6 His
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Description
ORFs cloned in this vector will be expressed in mammalian cells as a tagged protein with the C-terminal FLAG-6 His tags.
Such clones are the best for detection and purification of the transgene using anti-FLAG or anti-His antibodies.
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Vector Name
pcDNA3.1-3xFlag
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primers
T7: TAATACGACTCACTATAGGG
BGH: TAGAAGGCACAGTCGAGG -
Vector Length
5508
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Vector Type
Mammalian Expression Vector
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Promoter
CMV
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Antibiotic Resistance
Ampicillin
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Selection Marker
Neomycin
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Protein Tag
Flag
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Description
Mammalian vector for expressing C-terminal 3xFlag tagged proteins.
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