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Product Name
PPME1 Antibody (C-term)
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Description
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
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Tested applications
WB, ELISA
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Species reactivity
Human
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Alternative names
PME1 antibody; Protein phosphatase methylesterase 1 antibody; PME-1 antibody; PPME1 antibody; PME1 antibody
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Isotype
Rabbit IgG
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Preparation
This PPME1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 351-379 amino acids from the C-terminal region of human PPME1.
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Clonality
Polyclonal
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Formulation
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
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Storage instructions
Short term 4°C, long term aliquot and store at -20°C, avoid freeze thaw cycles. Store undiluted.
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Applications
WB: 1:1000
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Validations
PPME1 Antibody (C-term) (Cat. #134865) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the PPME1 antibody detected the PPME1 protein (arrow).
Western blot analysis of PPME1 (arrow) using rabbit polyclonal PPME1 Antibody (C-term) (Cat. #134865). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the PPME1 gene.
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Background
Demethylates proteins that have been reversibly carboxymethylated. Demethylates PPP2CB (in vitro) and PPP2CA. Binding to PPP2CA displaces the manganese ion and inactivates the enzyme.
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References
Related Products / Services
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"