Polyclonal antibody to Phospho-VASP-S157
WB, IHC, IP
Human, Mouse, Rat
VASP antibody; vasodilator-stimulated phosphoprotein antibody
Antigen: A synthetic phosphorylated peptide around S157 of human VASP (NP_003361.1).
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Store at -20℃. Avoid freeze / thaw cycles.
WB 1:500 - 1:2000
IHC 1:50 - 1:100
IP 1:50 - 1:100
Western blot - Phospho-VASP-S157 pAb
Western blot analysis of extracts of A431 cells,using Phospho-VASP-S157 antibody at 1:2000 dilution. A431 cells were treated by Forskolin (10uM) for 30 minutes after serum-starvation overnight.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% BSA.Detection: ECL Basic Kit .Exposure time: 1s.
Immunoprecipitation - Phospho-VASP-S157 pAb
Immunoprecipitation analysis of 200ug extracts of A-431 cells, using 3 ug Phospho-VASP-S157 pAb . Western blot was performed from the immunoprecipitate using Phospho-VASP-S157 pAb at a dilition of 1:1000. A-431 cells were treated by Forskolin (10 uM) at 37u2103 for 30 minutes after serum-starvation overnight.
Ena/VASP proteins are actin-associated proteins involved in a range of processes dependent on cytoskeleton remodeling and cell polarity such as axon guidance, lamellipodial and filopodial dynamics, platelet activation and cell migration. VASP promotes actin filament elongation. It protects the barbed end of growing actin filaments against capping and increases the rate of actin polymerization in the presence of capping protein. VASP stimulates actin filament elongation by promoting the transfer of profilin-bound actin monomers onto the barbed end of growing actin filaments. Plays a role in actin-based mobility of Listeria monocytogenes in host cells. Regulates actin dynamics in platelets and plays an important role in regulating platelet aggregation.
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