Polyclonal antibody to Phospho-STAT5-Y694
Human, Mouse, Rat
MGF antibody; STAT5 antibody
Antigen: A phospho specific peptide corresponding to residues surrounding Y694 of human STAT5.
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Store at -20℃. Avoid freeze / thaw cycles.
WB 1:500 - 1:2000
IHC 1:100 - 1:200
Western blot - Phospho-STAT5-Y694 pAb
Western blot analysis of extracts of K562 cells, using Phospho-STAT5-Y694 pAb at 1:1000 dilution.K-562 cells were treated by ATP(5 mM) at 30u2103 for 1 hour.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% BSA.Detection: ECL Basic Kit .Exposure time: 1s.
Carries out a dual signal transduction and activation of transcription. Mediates cellular responses to the cytokine KITLG/SCF and other growth factors. Mediates cellular responses to ERBB4. May mediate cellular responses to activated FGFR1, FGFR2, FGFR3 and FGFR4. Binds to the GAS element and activates PRL-induced transcription. Regulates the expression of milk proteins during lactation.
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