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Product Name
Phospho-SMAD3-T179 pAb
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Description
Polyclonal antibody to Phospho-SMAD3-T179
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Tested applications
WB, IHC
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Species reactivity
Human, Mouse, Rat
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Alternative names
SMAD3 antibody; HSPC193 antibody; HsT17436 antibody; JV15-2 antibody; LDS1C antibody; LDS3 antibody; MADH3 antibody; SMAD family member 3 antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: A synthetic phosphorylated peptide around T179 of human SMAD3 (NP_005893.1).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
IHC 1:50 - 1:200 -
Validations
Western blot - Phospho-SMAD3-T179 pAb
Western blot analysis of extracts of HeLa cell line, using Phospho-SMAD3-T179 antibody . The lane on the right is treated with the antigen-specific peptide.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% BSA.
Western blot - Phospho-SMAD3-T179 pAb
Western blot analysis of extracts of PC-12 cells, using Phospho-Smad2-S465/467/-Smad3-S423/425 pAb at 1:1000 dilution.PC-12 cells were treated by TNF-u03b1 (20 ng/ml) at 37u2103 for 30 minutes after serum-starvation overnight.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% BSA.Detection: ECL Basic Kit .Exposure time: 30s.
Immunohistochemistry - Phospho-SMAD3-T179 pAb
Immunohistochemistry of paraffin-embedded human colon using Phospho-SMAD3-T179 antibody at dilution of 1:200 (40x lens).
Immunohistochemistry - Phospho-SMAD3-T179 pAb
Immunohistochemistry of paraffin-embedded mouse kidney using Phospho-SMAD3-T179 antibody at dilution of 1:200 (40x lens).
Immunohistochemistry - Phospho-SMAD3-T179 pAb
Immunohistochemistry of paraffin-embedded rat liver using Phospho-SMAD3-T179 antibody at dilution of 1:200 (40x lens).
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Background
Receptor-regulated SMAD (R-SMAD) that is an intracellular signal transducer and transcriptional modulator activated by TGF-beta (transforming growth factor) and activin type 1 receptor kinases. Binds the TRE element in the promoter region of many genes that are regulated by TGF-beta and, on formation of the SMAD3/SMAD4 complex, activates transcription. Also can form a SMAD3/SMAD4/JUN/FOS complex at the AP-1/SMAD site to regulate TGF-beta-mediated transcription. Has an inhibitory effect on wound healing probably by modulating both growth and migration of primary keratinocytes and by altering the TGF-mediated chemotaxis of monocytes. This effect on wound healing appears to be hormone-sensitive. Regulator of chondrogenesis and osteogenesis and inhibits early healing of bone fractures. Positively regulates PDPK1 kinase activity by stimulating its dissociation from the 14-3-3 protein YWHAQ which acts as a negative regulator.
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References
Related Products / Services
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"