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  • Phospho-SMAD3-T179 pAb
  • Phospho-SMAD3-T179 pAb
  • Phospho-SMAD3-T179 pAb
  • Phospho-SMAD3-T179 pAb
  • Phospho-SMAD3-T179 pAb

Phospho-SMAD3-T179 pAb

Cat.#: 166480

Special Price 232.3 USD

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Product Information

  • Product Name
    Phospho-SMAD3-T179 pAb
  • Documents
  • Description
    Polyclonal antibody to Phospho-SMAD3-T179
  • Tested applications
    WB, IHC
  • Species reactivity
    Human, Mouse, Rat
  • Alternative names
    SMAD3 antibody; HSPC193 antibody; HsT17436 antibody; JV15-2 antibody; LDS1C antibody; LDS3 antibody; MADH3 antibody; SMAD family member 3 antibody
  • Isotype
    Rabbit IgG
  • Preparation
    Antigen: A synthetic phosphorylated peptide around T179 of human SMAD3 (NP_005893.1).
  • Clonality
    Polyclonal
  • Formulation
    PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
  • Storage instructions
    Store at -20℃. Avoid freeze / thaw cycles.
  • Applications
    WB 1:500 - 1:2000
    IHC 1:50 - 1:200
  • Validations

    Western blot - Phospho-SMAD3-T179 pAb

    Western blot - Phospho-SMAD3-T179 pAb

    Western blot analysis of extracts of HeLa cell line, using Phospho-SMAD3-T179 antibody . The lane on the right is treated with the antigen-specific peptide.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% BSA.

    Western blot - Phospho-SMAD3-T179 pAb

    Western blot - Phospho-SMAD3-T179 pAb

    Western blot analysis of extracts of PC-12 cells, using Phospho-Smad2-S465/467/-Smad3-S423/425 pAb at 1:1000 dilution.PC-12 cells were treated by TNF-u03b1 (20 ng/ml) at 37u2103 for 30 minutes after serum-starvation overnight.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% BSA.Detection: ECL Basic Kit .Exposure time: 30s.

    Immunohistochemistry - Phospho-SMAD3-T179 pAb

    Immunohistochemistry - Phospho-SMAD3-T179 pAb

    Immunohistochemistry of paraffin-embedded human colon using Phospho-SMAD3-T179 antibody at dilution of 1:200 (40x lens).

    Immunohistochemistry - Phospho-SMAD3-T179 pAb

    Immunohistochemistry - Phospho-SMAD3-T179 pAb

    Immunohistochemistry of paraffin-embedded mouse kidney using Phospho-SMAD3-T179 antibody at dilution of 1:200 (40x lens).

    Immunohistochemistry - Phospho-SMAD3-T179 pAb

    Immunohistochemistry - Phospho-SMAD3-T179 pAb

    Immunohistochemistry of paraffin-embedded rat liver using Phospho-SMAD3-T179 antibody at dilution of 1:200 (40x lens).

  • Background
    Receptor-regulated SMAD (R-SMAD) that is an intracellular signal transducer and transcriptional modulator activated by TGF-beta (transforming growth factor) and activin type 1 receptor kinases. Binds the TRE element in the promoter region of many genes that are regulated by TGF-beta and, on formation of the SMAD3/SMAD4 complex, activates transcription. Also can form a SMAD3/SMAD4/JUN/FOS complex at the AP-1/SMAD site to regulate TGF-beta-mediated transcription. Has an inhibitory effect on wound healing probably by modulating both growth and migration of primary keratinocytes and by altering the TGF-mediated chemotaxis of monocytes. This effect on wound healing appears to be hormone-sensitive. Regulator of chondrogenesis and osteogenesis and inhibits early healing of bone fractures. Positively regulates PDPK1 kinase activity by stimulating its dissociation from the 14-3-3 protein YWHAQ which acts as a negative regulator.
  • References

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"