Monoclonal antibody to Phospho-Smad3-S423/S425
WB, IHC, IF
SMAD3 antibody; HSPC193 antibody; HsT17436 antibody; JV15-2 antibody; LDS1C antibody; LDS3 antibody; MADH3 antibody; SMAD family member 3 antibody
Antigen: A phospho specific peptide corresponding to residues surrounding S423/S425 of human Smad3
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Store at -20℃. Avoid freeze / thaw cycles.
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
Western blot - Phospho-Smad3-S423/S425 mAb
Western blot analysis of extracts of various cell lines, using Phospho-Smad3-S423/S425 antibody at dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.
Immunohistochemistry - Phospho-Smad3-S423/S425 mAb
Immunohistochemistry of paraffin-embedded mouse skin using Phospho-Smad3-S423/S425 antibody at dilution of 1:100 (40x lens).
Immunofluorescence - Phospho-Smad3-S423/S425 mAb
Immunofluorescence analysis of A431 cells using Phospho-Smad3-S423/S425 antibody .
Receptor-regulated SMAD (R-SMAD) that is an intracellular signal transducer and transcriptional modulator activated by TGF-beta (transforming growth factor) and activin type 1 receptor kinases. Binds the TRE element in the promoter region of many genes that are regulated by TGF-beta and, on formation of the SMAD3/SMAD4 complex, activates transcription. Also can form a SMAD3/SMAD4/JUN/FOS complex at the AP-1/SMAD site to regulate TGF-beta-mediated transcription. Has an inhibitory effect on wound healing probably by modulating both growth and migration of primary keratinocytes and by altering the TGF-mediated chemotaxis of monocytes. This effect on wound healing appears to be hormone-sensitive. Regulator of chondrogenesis and osteogenesis and inhibits early healing of bone fractures. Positively regulates PDPK1 kinase activity by stimulating its dissociation from the 14-3-3 protein YWHAQ which acts as a negative regulator.
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