Phospho-Smad3-S423/S425 mAb

Phospho-Smad3-S423/S425 mAb

• DataSheet
• Material Safety Data Sheets (MSDS)

Monoclonal antibody to Phospho-Smad3-S423/S425

WB, IHC, IF

Human, Mouse

SMAD3 antibody; HSPC193 antibody; HsT17436 antibody; JV15-2 antibody; LDS1C antibody; LDS3 antibody; MADH3 antibody; SMAD family member 3 antibody

Rabbit IgG

Antigen: A phospho specific peptide corresponding to residues surrounding S423/S425 of human Smad3

Monoclonal

PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Store at -20℃. Avoid freeze / thaw cycles.

WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200

Receptor-regulated SMAD (R-SMAD) that is an intracellular signal transducer and transcriptional modulator activated by TGF-beta (transforming growth factor) and activin type 1 receptor kinases. Binds the TRE element in the promoter region of many genes that are regulated by TGF-beta and, on formation of the SMAD3/SMAD4 complex, activates transcription. Also can form a SMAD3/SMAD4/JUN/FOS complex at the AP-1/SMAD site to regulate TGF-beta-mediated transcription. Has an inhibitory effect on wound healing probably by modulating both growth and migration of primary keratinocytes and by altering the TGF-mediated chemotaxis of monocytes. This effect on wound healing appears to be hormone-sensitive. Regulator of chondrogenesis and osteogenesis and inhibits early healing of bone fractures. Positively regulates PDPK1 kinase activity by stimulating its dissociation from the 14-3-3 protein YWHAQ which acts as a negative regulator.