Phospho-Smad2-S465/467 + Smad3-S423/425 pAb
Polyclonal antibody to Phospho-Smad2-S465/467 + Smad3-S423/425
Antigen: A synthetic phosphorylated peptide around S465 & S467 of human Smad2 (NP_005892.1).
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Store at -20℃. Avoid freeze / thaw cycles.
WB 1:500 - 1:2000
Western blot - Phospho-Smad2-S465/467 + Smad3-S423/425 pAb
Western blot analysis of extracts of HeLa cells, using Phospho-Smad2-S465/467/-Smad3-S423/425 pAb at 1:1000 dilution or Smad2/Smad3 .HeLa cells were treated by TGF-u03b2 (10ng/ml) for 30 minutes after serum-starvation overnight.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% BSA.Detection: ECL Basic Kit .Exposure time: 1s.
Receptor-regulated SMAD (R-SMAD) that is an intracellular signal transducer and transcriptional modulator activated by TGF-beta (transforming growth factor) and activin type 1 receptor kinases. Binds the TRE element in the promoter region of many genes that are regulated by TGF-beta and, on formation of the SMAD2/SMAD4 complex, activates transcription. May act as a tumor suppressor in colorectal carcinoma. Positively regulates PDPK1 kinase activity by stimulating its dissociation from the 14-3-3 protein YWHAQ which acts as a negative regulator.
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