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  • Phospho-Smad2-S465/467 + Smad3-S423/425 pAb

Phospho-Smad2-S465/467 + Smad3-S423/425 pAb

Cat.#: 166478

Special Price 232.3 USD

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Product Information

  • Product Name
    Phospho-Smad2-S465/467 + Smad3-S423/425 pAb
  • Documents
  • Description
    Polyclonal antibody to Phospho-Smad2-S465/467 + Smad3-S423/425
  • Tested applications
    WB
  • Species reactivity
    Human, Mouse
  • Alternative names
    antibody
  • Isotype
    Rabbit IgG
  • Preparation
    Antigen: A synthetic phosphorylated peptide around S465 & S467 of human Smad2 (NP_005892.1).
  • Clonality
    Polyclonal
  • Formulation
    PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
  • Storage instructions
    Store at -20℃. Avoid freeze / thaw cycles.
  • Applications
    WB 1:500 - 1:2000
  • Validations

    Western blot - Phospho-Smad2-S465/467 + Smad3-S423/425 pAb

    Western blot - Phospho-Smad2-S465/467 + Smad3-S423/425 pAb

    Western blot analysis of extracts of HeLa cells, using Phospho-Smad2-S465/467/-Smad3-S423/425 pAb at 1:1000 dilution or Smad2/Smad3 .HeLa cells were treated by TGF-u03b2 (10ng/ml) for 30 minutes after serum-starvation overnight.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% BSA.Detection: ECL Basic Kit .Exposure time: 1s.

  • Background
    Receptor-regulated SMAD (R-SMAD) that is an intracellular signal transducer and transcriptional modulator activated by TGF-beta (transforming growth factor) and activin type 1 receptor kinases. Binds the TRE element in the promoter region of many genes that are regulated by TGF-beta and, on formation of the SMAD2/SMAD4 complex, activates transcription. May act as a tumor suppressor in colorectal carcinoma. Positively regulates PDPK1 kinase activity by stimulating its dissociation from the 14-3-3 protein YWHAQ which acts as a negative regulator.
  • References

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"