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Product Name
Phospho-Smad1-S463/465 + Smad5-S463/465 + Smad9-S465/467 pAb
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Description
Polyclonal antibody to Phospho-Smad1-S463/465 + Smad5-S463/465 + Smad9-S465/467
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Tested applications
WB
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Species reactivity
Human, Mouse
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Alternative names
antibody
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Isotype
Rabbit IgG
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Preparation
Antigen:
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
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Validations
Western blot - Phospho-Smad1-S463/465 + Smad5-S463/465 + Smad9-S465/467 pAb
Western blot analysis of extracts of HeLa and NIH/3T3 cells, using Phospho-Smad1(Ser463/465)/Smad5(Ser463/465)/Smad9(Ser465/467) pAb at 1:1000 dilution.HeLa and NIH/3T3 cells were treated by ATP(5 mM) at 30u2103 for 1 hour.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% BSA.Detection: ECL Basic Kit .Exposure time: 60s.
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Background
Transcriptional modulator activated by BMP (bone morphogenetic proteins) type 1 receptor kinase. SMAD1 is a receptor-regulated SMAD (R-SMAD). SMAD1/OAZ1/PSMB4 complex mediates the degradation of the CREBBP/EP300 repressor SNIP1. May act synergistically with SMAD4 and YY1 in bone morphogenetic protein (BMP)-mediated cardiac-specific gene expression.
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References
Related Products / Services
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"