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  • Phospho-SHC1-Y349 pAb
  • Phospho-SHC1-Y349 pAb

Phospho-SHC1-Y349 pAb

Cat.#: 166379

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Product Information

  • Product Name
    Phospho-SHC1-Y349 pAb
  • Documents
  • Description
    Polyclonal antibody to Phospho-SHC1-Y349
  • Tested applications
    WB, IF
  • Species reactivity
    Human, Mouse
  • Alternative names
    SHC1 antibody; SHC antibody; SHCA antibody; SHC adaptor protein 1 antibody
  • Isotype
    Rabbit IgG
  • Preparation
    Antigen: A phospho specific peptide corresponding to residues surrounding Y349 of human SHC1
  • Clonality
    Polyclonal
  • Formulation
    PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
  • Storage instructions
    Store at -20℃. Avoid freeze / thaw cycles.
  • Applications
    WB 1:500 - 1:2000
    IF 1:100 - 1:200
  • Validations

    Western blot - Phospho-SHC1-Y349 pAb

    Western blot - Phospho-SHC1-Y349 pAb

    Western blot analysis of extracts from HepG2 cells using Phospho-SHC1-Y349 antibody .Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% BSA.

    Immunofluorescence - Phospho-SHC1-Y349 pAb

    Immunofluorescence - Phospho-SHC1-Y349 pAb

    Immunofluorescence analysis of methanol-fixed HeLa cells using Phospho-SHC1-Y349 antibody .

  • Background
    Signaling adapter that couples activated growth factor receptors to signaling pathways. Participates in a signaling cascade initiated by activated KIT and KITLG/SCF. Isoform p46Shc and isoform p52Shc, once phosphorylated, couple activated receptor tyrosine kinases to Ras via the recruitment of the GRB2/SOS complex and are implicated in the cytoplasmic propagation of mitogenic signals. Isoform p46Shc and isoform p52Shc may thus function as initiators of the Ras signaling cascade in various non-neuronal systems. Isoform p66Shc does not mediate Ras activation, but is involved in signal transduction pathways that regulate the cellular response to oxidative stress and life span. Isoform p66Shc acts as a downstream target of the tumor suppressor p53 and is indispensable for the ability of stress-activated p53 to induce elevation of intracellular oxidants, cytochrome c release and apoptosis. The expression of isoform p66Shc has been correlated with life span (By similarity). Participates in signaling downstream of the angiopoietin receptor TEK/TIE2, and plays a role in the regulation of endothelial cell migration and sprouting angiogenesis.
  • References

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"