Polyclonal antibody to Phospho-RPS6-S240/244
RPS6 antibody; S6 antibody; ribosomal protein S6 antibody
Antigen: A synthetic phosphorylated peptide around S240 & S244 of human RPS6 (NP_001001.2).
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Store at -20℃. Avoid freeze / thaw cycles.
WB 1:500 - 1:2000
IP 1:50 - 1:100
Western blot - Phospho-RPS6-S240/244 pAb
Western blot analysis of extracts of various cell lines, using Phospho-RPS6KB1-S240/244 antibody at 1:1000 dilution or RPS6 antibody . HeLa cells were treated by 10% FBS at 37u2103 for 30 minutes after serum-starvation overnight. NIH/3T3 cells were treated by 10% FBS at 37u2103 for 30 minutes after serum-starvation overnight.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% BSA.Detection: ECL Basic Kit .Exposure time: 1s.
Immunoprecipitation - Phospho-RPS6-S240/244 pAb
Immunoprecipitation analysis of 200ug extracts of 293T cells, using 3 ug Phospho-RPS6-S240/244 pAb . Western blot was performed from the immunoprecipitate using Phospho-RPS6-S240/244 pAb at a dilition of 1:1000. 293T cells were treated by PMA/TPA (200 nM) at 37u2103 for 30 minutes after serum-starvation overnight.
May play an important role in controlling cell growth and proliferation through the selective translation of particular classes of mRNA.
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