Polyclonal antibody to Phospho-RPS6-S235/236
Human, Mouse, Rat
RPS6 antibody; S6 antibody; ribosomal protein S6 antibody
Antigen: A synthetic phosphorylated peptide around S235 & S236 of human RPS6 (NP_001001.2).
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Store at -20℃. Avoid freeze / thaw cycles.
WB 1:500 - 1:2000
Western blot - Phospho-RPS6-S235/236 pAb
Western blot analysis of extracts of HeLa and 293T cells, using Phospho-RPS6KB1-S235/236 antibody at 1:1000 dilution. HeLa cells were treated by EGF (100ng/ml) for 30 minutes after serum-starvation overnight. 293T cells were treated by PMA/TPA (200nM) for 30 minutes after serum-starvation overnight.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% BSA.
May play an important role in controlling cell growth and proliferation through the selective translation of particular classes of mRNA.
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