Polyclonal antibody to Phospho-PTPN6-Y564
PTPN6 antibody; HCP antibody; HCPH antibody; HPTP1C antibody; PTP-1C antibody; SH-PTP1 antibody; SHP-1 antibody; SHP-1L antibody; SHP1 antibody; tyrosine-protein phosphatase non-receptor type 6 antibody
Antigen: A synthetic phosphorylated peptide around Y564 of human PTPN6 (NP_002822.2).
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Store at -20℃. Avoid freeze / thaw cycles.
WB 1:500 - 1:2000
Western blot - Phospho-PTPN6-Y564 pAb
Western blot analysis of extracts of HeLa and RAW 264.7 cells, using Phospho-PTPN6-Y564 antibody at 1:2000 dilution. HeLa cell lysates were treated by CIP at 37u00b0C for 1 hour.RAW 264.7 cell lysates were treated by CIP at 37u00b0C for 1 hour.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% BSA.Detection: ECL Enhanced Kit .Exposure time: 3min.
Modulates signaling by tyrosine phosphorylated cell surface receptors such as KIT and the EGF receptor/EGFR. The SH2 regions may interact with other cellular components to modulate its own phosphatase activity against interacting substrates. Together with MTUS1, induces UBE2V2 expression upon angiotensin II stimulation. Plays a key role in hematopoiesis.
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