Polyclonal antibody to Phospho-PTPN11-Y542
Human, Mouse, Rat
PTPN11 antibody; BPTP3 antibody; CFC antibody; JMML antibody; METCDS antibody; NS1 antibody; PTP-1D antibody; PTP2C antibody; SH-PTP2 antibody; SH-PTP3 antibody; SHP2 antibody; tyrosine-protein phosphatase non-receptor type 11 antibody
Antigen: A phospho specific peptide corresponding to residues surrounding Y542 of human PTPN11
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Store at -20℃. Avoid freeze / thaw cycles.
WB 1:500 - 1:2000
IHC 1:50 - 1:100
Western blot - Phospho-PTPN11-Y542 pAb
Western blot analysis of extracts from 293 cells using Phospho-PTPN11-Y542 antibody .Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% BSA.
Immunohistochemistry - Phospho-PTPN11-Y542 pAb
Immunohistochemistry of paraffin-embedded human breast carcinoma using Phospho-PTPN11-Y542 antibody .
Acts downstream of various receptor and cytoplasmic protein tyrosine kinases to participate in the signal transduction from the cell surface to the nucleus. Positively regulates MAPK signal transduction pathway. Dephosphorylates GAB1, ARHGAP35 and EGFR. Dephosphorylates ROCK2 at 'Tyr-722' resulting in stimulatation of its RhoA binding activity. Dephosphorylates CDC73.
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