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  • Phospho-PPP1CA-T320 pAb
  • Phospho-PPP1CA-T320 pAb
  • Phospho-PPP1CA-T320 pAb
  • Phospho-PPP1CA-T320 pAb
  • Phospho-PPP1CA-T320 pAb
  • Phospho-PPP1CA-T320 pAb

Phospho-PPP1CA-T320 pAb

Cat.#: 166522

Special Price 232.3 USD

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Product Information

  • Product Name
    Phospho-PPP1CA-T320 pAb
  • Documents
  • Description
    Polyclonal antibody to Phospho-PPP1CA-T320
  • Tested applications
    WB, IHC
  • Species reactivity
    Human, Mouse, Rat
  • Alternative names
    PPP1CA antibody; PP-1A antibody; PP1A antibody; PP1alpha antibody; PPP1A antibody; protein phosphatase 1 catalytic subunit alpha antibody
  • Isotype
    Rabbit IgG
  • Preparation
    Antigen: A phospho specific peptide corresponding to residues surrounding T320 of human PPP1CA
  • Clonality
    Polyclonal
  • Formulation
    PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
  • Storage instructions
    Store at -20℃. Avoid freeze / thaw cycles.
  • Applications
    WB 1:500 - 1:2000
    IHC 1:50 - 1:100
  • Validations

    Western blot - Phospho-PPP1CA-T320 pAb

    Western blot - Phospho-PPP1CA-T320 pAb

    Western blot analysis of extracts of various cell lines, using Phospho-PPP1CA-T320 antibody at 1:2000 dilution or PPP1CA antibody . NIH/3T3 cells were treated by Calyculin A (100 nM) at 37u2103 for 30 minutes after serum-starvation overnight.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% BSA.Detection: ECL Basic Kit .Exposure time: 30s.

    Immunohistochemistry - Phospho-PPP1CA-T320 pAb

    Immunohistochemistry - Phospho-PPP1CA-T320 pAb

    Immunohistochemistry of paraffin-embedded rat lung using Phospho-PPP1CA-T320 antibody at dilution of 1:100 (40x lens).

    Immunohistochemistry - Phospho-PPP1CA-T320 pAb

    Immunohistochemistry - Phospho-PPP1CA-T320 pAb

    Immunohistochemistry of paraffin-embedded human lung cancer using Phospho-PPP1CA-T320 antibody at dilution of 1:100 (40x lens).

    Immunohistochemistry - Phospho-PPP1CA-T320 pAb

    Immunohistochemistry - Phospho-PPP1CA-T320 pAb

    Immunohistochemistry of paraffin-embedded human appendix using Phospho-PPP1CA-T320 antibody at dilution of 1:100 (40x lens).

    Immunohistochemistry - Phospho-PPP1CA-T320 pAb

    Immunohistochemistry - Phospho-PPP1CA-T320 pAb

    Immunohistochemistry of paraffin-embedded human placenta using Phospho-PPP1CA-T320 antibody at dilution of 1:100 (40x lens).

    Immunohistochemistry - Phospho-PPP1CA-T320 pAb

    Immunohistochemistry - Phospho-PPP1CA-T320 pAb

    Immunohistochemistry of paraffin-embedded human gastric cancer using Phospho-PPP1CA-T320 antibody at dilution of 1:100 (40x lens).

  • Background
    Protein phosphatase that associates with over 200 regulatory proteins to form highly specific holoenzymes which dephosphorylate hundreds of biological targets. Protein phosphatase 1 (PP1) is essential for cell division, and participates in the regulation of glycogen metabolism, muscle contractility and protein synthesis. Involved in regulation of ionic conductances and long-term synaptic plasticity. May play an important role in dephosphorylating substrates such as the postsynaptic density-associated Ca(2+)/calmodulin dependent protein kinase II. Component of the PTW/PP1 phosphatase complex, which plays a role in the control of chromatin structure and cell cycle progression during the transition from mitosis into interphase. Regulates NEK2 function in terms of kinase activity and centrosome number and splitting, both in the presence and absence of radiation-induced DNA damage. Regulator of neural tube and optic fissure closure, and enteric neural crest cell (ENCCs) migration during development. In balance with CSNK1D and CSNK1E, determines the circadian period length, through the regulation of the speed and rhythmicity of PER1 and PER2 phosphorylation. May dephosphorylate CSNK1D and CSNK1E. Dephosphorylates the 'Ser-418' residue of FOXP3 in regulatory T-cells (Treg) from patients with rheumatoid arthritis, thereby inactivating FOXP3 and rendering Treg cells functionally defective. Dephosphorylates CENPA. Dephosphorylates the 'Ser-139' residue of ATG16L1 causing dissociation of ATG12-ATG5-ATG16L1 complex, thereby inhibiting autophagy.
  • References

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"