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  • Phospho-POLR2A-S2 pAb
  • Phospho-POLR2A-S2 pAb
  • Phospho-POLR2A-S2 pAb

Phospho-POLR2A-S2 pAb

Cat.#: 166509

Special Price 232.3 USD

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Product Information

  • Product Name
    Phospho-POLR2A-S2 pAb
  • Documents
  • Description
    Polyclonal antibody to Phospho-POLR2A-S2
  • Tested applications
    WB, IF, IP
  • Species reactivity
    Human
  • Alternative names
    POLR2A antibody; POLR2 antibody; POLRA antibody; RPB1 antibody; RPBh1 antibody; RPO2 antibody; RPOL2 antibody; RpIILS antibody; hRPB220 antibody; hsRPB1 antibody; RNA polymerase II subunit A antibody
  • Isotype
    Rabbit IgG
  • Preparation
    Antigen: A phospho specific peptide corresponding to residues surrounding S2 of human POLR2A
  • Clonality
    Polyclonal
  • Formulation
    PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
  • Storage instructions
    Store at -20℃. Avoid freeze / thaw cycles.
  • Applications
    WB 1:1000 - 1:2000
    IF 1:50 - 1:100
    IP 1:50 - 1:100
  • Validations

    Western blot - Phospho-POLR2A-S2 pAb

    Western blot - Phospho-POLR2A-S2 pAb

    Western blot analysis of extracts of 293T cells, using Phospho-POLR2A-S2 antibody at 1:1000 dilution. 293T cell lysate were treated by CIP (20ul CIP for each 400ul cell lysate) at 37u2103 for 1 hour or treated by Doxorubicin (0.5uM) for 24 hours.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% BSA.Detection: ECL Basic Kit .Exposure time: 1s.

    Immunofluorescence - Phospho-POLR2A-S2 pAb

    Immunofluorescence - Phospho-POLR2A-S2 pAb

    Immunofluorescence analysis of HeLa cells using Phospho-POLR2A-S2 antibody at dilution of 1:100. HeLa cells were treated by nocodazole (50 ng/ml) at 37u2103 for 20 hours (left). Blue: DAPI for nuclear staining.

    Immunoprecipitation - Phospho-POLR2A-S2 pAb

    Immunoprecipitation - Phospho-POLR2A-S2 pAb

    Immunoprecipitation analysis of 200ug extracts of MCF-7 cells, using 3 ug Phospho-POLR2A-S2 pAb . Western blot was performed from the immunoprecipitate using Phospho-POLR2A-S2 pAb at a dilition of 1:1000. MCF7 cells were treated by nocodazole (50 ng/ml) at 37u2103 for 20 hours.

  • Background
    DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Largest and catalytic component of RNA polymerase II which synthesizes mRNA precursors and many functional non-coding RNAs. Forms the polymerase active center together with the second largest subunit. Pol II is the central component of the basal RNA polymerase II transcription machinery. It is composed of mobile elements that move relative to each other. RPB1 is part of the core element with the central large cleft, the clamp element that moves to open and close the cleft and the jaws that are thought to grab the incoming DNA template. At the start of transcription, a single-stranded DNA template strand of the promoter is positioned within the central active site cleft of Pol II. A bridging helix emanates from RPB1 and crosses the cleft near the catalytic site and is thought to promote translocation of Pol II by acting as a ratchet that moves the RNA-DNA hybrid through the active site by switching from straight to bent conformations at each step of nucleotide addition. During transcription elongation, Pol II moves on the template as the transcript elongates. Elongation is influenced by the phosphorylation status of the C-terminal domain (CTD) of Pol II largest subunit (RPB1), which serves as a platform for assembly of factors that regulate transcription initiation, elongation, termination and mRNA processing. Regulation of gene expression levels depends on the balance between methylation and acetylation levels of tha CTD-lysines (By similarity). Initiation or early elongation steps of transcription of growth-factors-induced immediate early genes are regulated by the acetylation status of the CTD. Methylation and dimethylation have a repressive effect on target genes expression (By similarity).; (Microbial infection) Acts as an RNA-dependent RNA polymerase when associated with small delta antigen of Hepatitis delta virus, acting both as a replicate and transcriptase for the viral RNA circular genome.
  • References

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"