Polyclonal antibody to Phospho-PLCG2-Y759
PLCG2 antibody; APLAID antibody; FCAS3 antibody; PLC-IV antibody; PLC-gamma-2 antibody; phospholipase C gamma 2 antibody
Antigen: A synthetic phosphorylated peptide around Y759 of human PLCG2 (NP_002652.2).
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Store at -20℃. Avoid freeze / thaw cycles.
WB 1:500 - 1:2000
Western blot - Phospho-PLCG2-Y759 pAb
Western blot analysis of extracts of Raji cells, using Phospho-PLCG2-Y759 antibody at 1:2000 dilution or PLCG2 antibody . Raji cells were treated by Pervanadate (1 mM) at 37u2103 for 30 minutes.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% BSA.Detection: ECL Basic Kit .Exposure time: 1s.
The production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) is mediated by activated phosphatidylinositol-specific phospholipase C enzymes. It is a crucial enzyme in transmembrane signaling.
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