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  • Phospho-PLCG2-Y1217 pAb
  • Phospho-PLCG2-Y1217 pAb

Phospho-PLCG2-Y1217 pAb

Cat.#: 166527

Special Price 232.3 USD

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Product Information

  • Product Name
    Phospho-PLCG2-Y1217 pAb
  • Documents
  • Description
    Polyclonal antibody to Phospho-PLCG2-Y1217
  • Tested applications
    WB, IHC
  • Species reactivity
    Human, Mouse
  • Alternative names
    PLCG2 antibody; APLAID antibody; FCAS3 antibody; PLC-IV antibody; PLC-gamma-2 antibody; phospholipase C gamma 2 antibody
  • Isotype
    Rabbit IgG
  • Preparation
    Antigen: A synthetic phosphorylated peptide around Y1217 of human PLCG2 (NP_002652.2).
  • Clonality
    Polyclonal
  • Formulation
    PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
  • Storage instructions
    Store at -20℃. Avoid freeze / thaw cycles.
  • Applications
    WB 1:500 - 1:2000
    IHC 1:50 - 1:200
  • Validations

    Western blot - Phospho-PLCG2-Y1217 pAb

    Western blot - Phospho-PLCG2-Y1217 pAb

    Western blot analysis of extracts of Raji cells, using Phospho-PLCG2-Y1217 antibody at 1:1000 dilution or PLCG2 antibody . Raji cells were treated by Pervanadate (1 mM) at 37u2103 for 30 minutes.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% BSA.Detection: ECL Basic Kit .Exposure time: 1s.

    Immunohistochemistry - Phospho-PLCG2-Y1217 pAb

    Immunohistochemistry - Phospho-PLCG2-Y1217 pAb

    Immunohistochemistry of paraffin-embedded mouse spleen using Phospho-PLCG2-Y1217 antibody at dilution of 1:100 (40x lens).

  • Background
    The production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) is mediated by activated phosphatidylinositol-specific phospholipase C enzymes. It is a crucial enzyme in transmembrane signaling.
  • References

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"