Polyclonal antibody to Phospho-PLCG1-S1248
Human, Mouse, Rat
PLCG1 antibody; NCKAP3 antibody; PLC-II antibody; PLC1 antibody; PLC148 antibody; PLCgamma1 antibody; phospholipase C gamma 1 antibody
Antigen: A synthetic phosphorylated peptide around S1248 of human PLCG1 (NP_002651.2).
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Store at -20℃. Avoid freeze / thaw cycles.
WB 1:500 - 1:2000
IHC 1:10 - 1:100
Western blot - Phospho-PLCG1-S1248 pAb
Western blot analysis of extracts of Jurkat cells, using Phospho-PLCG1-S1248 antibody at dilution or PLCG1 antibody . Jurkat cells were treated by PMA/TPA (200 nM) at 37u2103 for 10 minutes.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% BSA.Detection: ECL Basic Kit .Exposure time: 90s.
Immunohistochemistry - Phospho-PLCG1-S1248 pAb
Immunohistochemistry of paraffin-embedded human placenta using Phospho-PLCG1-S1248 antibody at dilution of 1:100 (40x lens).
Mediates the production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3). Plays an important role in the regulation of intracellular signaling cascades. Becomes activated in response to ligand-mediated activation of receptor-type tyrosine kinases, such as PDGFRA, PDGFRB, FGFR1, FGFR2, FGFR3 and FGFR4. Plays a role in actin reorganization and cell migration.
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