Phospho-p27 KIP 1-T187 pAb
Polyclonal antibody to Phospho-p27 KIP 1-T187
Human, Mouse, Rat
CDKN1B antibody; CDKN4 antibody; KIP1 antibody; MEN1B antibody; MEN4 antibody; P27KIP1 antibody; cyclin-dependent kinase inhibitor 1B antibody
Antigen: A phospho specific peptide corresponding to residues surrounding T187 of human p27 KIP 1
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Store at -20℃. Avoid freeze / thaw cycles.
WB 1:500 - 1:1000
IHC 1:50 - 1:100
Western blot - Phospho-p27 KIP 1-T187 pAb
Western blot analysis of extracts from HeLa cells, using Phospho-p27 KIP 1-T187 antibody .Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% BSA.
Immunohistochemistry - Phospho-p27 KIP 1-T187 pAb
Immunohistochemistry of paraffin-embedded human breast carcinoma tissue, using Phospho-p27 KIP 1-T187 antibody .
Important regulator of cell cycle progression. Inhibits the kinase activity of CDK2 bound to cyclin A, but has little inhibitory activity on CDK2 bound to SPDYA. Involved in G1 arrest. Potent inhibitor of cyclin E- and cyclin A-CDK2 complexes. Forms a complex with cyclin type D-CDK4 complexes and is involved in the assembly, stability, and modulation of CCND1-CDK4 complex activation. Acts either as an inhibitor or an activator of cyclin type D-CDK4 complexes depending on its phosphorylation state and/or stoichometry.
Related Products / Services
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"