Polyclonal antibody to Phospho-MYH9-S1943
Human, Mouse, Rat
MYH9 antibody; BDPLT6 antibody; DFNA17 antibody; EPSTS antibody; FTNS antibody; MHA antibody; NMHC-II-A antibody; NMMHC-IIA antibody; NMMHCA antibody; myosin-9 antibody
Antigen: A synthetic phosphorylated peptide around S1943 of human MYH9 (NP_002464.1).
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Store at -20℃. Avoid freeze / thaw cycles.
WB 1:500 - 1:2000
Western blot - Phospho-MYH9-S1943 pAb
Western blot analysis of extracts of 293T cells, using Phospho-MYH9-S1943 antibody at 1:2000 dilution. 293T cell lysates were treated by CIP at 37u00b0C for 1 hour.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% BSA.Detection: ECL Enhanced Kit .Exposure time: 30s.
Cellular myosin that appears to play a role in cytokinesis, cell shape, and specialized functions such as secretion and capping. During cell spreading, plays an important role in cytoskeleton reorganization, focal contacts formation (in the margins but not the central part of spreading cells), and lamellipodial retraction; this function is mechanically antagonized by MYH10.
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