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  • Phospho-MDM2-S166 pAb
  • Phospho-MDM2-S166 pAb
  • Phospho-MDM2-S166 pAb

Phospho-MDM2-S166 pAb

Cat.#: 166391

Special Price 232.3 USD

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Product Information

  • Product Name
    Phospho-MDM2-S166 pAb
  • Documents
  • Description
    Polyclonal antibody to Phospho-MDM2-S166
  • Tested applications
    WB, IHC, IF
  • Species reactivity
    Human
  • Alternative names
    MDM2 antibody; ACTFS antibody; HDMX antibody; hdm2 antibody; MDM2 proto-oncogene antibody
  • Isotype
    Rabbit IgG
  • Preparation
    Antigen: A phospho specific peptide corresponding to residues surrounding S166 of human MDM2
  • Clonality
    Polyclonal
  • Formulation
    PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
  • Storage instructions
    Store at -20℃. Avoid freeze / thaw cycles.
  • Applications
    WB 1:500 - 1:2000
    IHC 1:50 - 1:100
    IF 1:100 - 1:200
  • Validations

    Western blot - Phospho-MDM2-S166 pAb

    Western blot - Phospho-MDM2-S166 pAb

    Western blot analysis of extracts from 293 cells using Phospho-MDM2-S166 antibody .Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% BSA.

    Immunohistochemistry - Phospho-MDM2-S166 pAb

    Immunohistochemistry - Phospho-MDM2-S166 pAb

    Immunohistochemistry of paraffin-embedded human breast carcinoma using Phospho-MDM2-S166 antibody .

    Immunofluorescence - Phospho-MDM2-S166 pAb

    Immunofluorescence - Phospho-MDM2-S166 pAb

    Immunofluorescence analysis of methanol-fixed HeLa cells using Phospho-MDM2-S166 antibody .

  • Background
    E3 ubiquitin-protein ligase that mediates ubiquitination of p53/TP53, leading to its degradation by the proteasome. Inhibits p53/TP53- and p73/TP73-mediated cell cycle arrest and apoptosis by binding its transcriptional activation domain. Also acts as a ubiquitin ligase E3 toward itself and ARRB1. Permits the nuclear export of p53/TP53. Promotes proteasome-dependent ubiquitin-independent degradation of retinoblastoma RB1 protein. Inhibits DAXX-mediated apoptosis by inducing its ubiquitination and degradation. Component of the TRIM28/KAP1-MDM2-p53/TP53 complex involved in stabilizing p53/TP53. Also component of the TRIM28/KAP1-ERBB4-MDM2 complex which links growth factor and DNA damage response pathways. Mediates ubiquitination and subsequent proteasome degradation of DYRK2 in nucleus. Ubiquitinates IGF1R and SNAI1 and promotes them to proteasomal degradation. Ubiquitinates DCX, leading to DCX degradation and reduction of the dendritic spine density of olfactory bulb granule cells (By similarity). Ubiquitinates DLG4, leading to proteasomal degradation of DLG4 which is required for AMPA receptor endocytosis (By similarity).
  • References

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"