Polyclonal antibody to Phospho-MDM2-S166
WB, IHC, IF
MDM2 antibody; ACTFS antibody; HDMX antibody; hdm2 antibody; MDM2 proto-oncogene antibody
Antigen: A phospho specific peptide corresponding to residues surrounding S166 of human MDM2
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Store at -20℃. Avoid freeze / thaw cycles.
WB 1:500 - 1:2000
IHC 1:50 - 1:100
IF 1:100 - 1:200
Western blot - Phospho-MDM2-S166 pAb
Western blot analysis of extracts from 293 cells using Phospho-MDM2-S166 antibody .Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% BSA.
Immunohistochemistry - Phospho-MDM2-S166 pAb
Immunohistochemistry of paraffin-embedded human breast carcinoma using Phospho-MDM2-S166 antibody .
Immunofluorescence - Phospho-MDM2-S166 pAb
Immunofluorescence analysis of methanol-fixed HeLa cells using Phospho-MDM2-S166 antibody .
E3 ubiquitin-protein ligase that mediates ubiquitination of p53/TP53, leading to its degradation by the proteasome. Inhibits p53/TP53- and p73/TP73-mediated cell cycle arrest and apoptosis by binding its transcriptional activation domain. Also acts as a ubiquitin ligase E3 toward itself and ARRB1. Permits the nuclear export of p53/TP53. Promotes proteasome-dependent ubiquitin-independent degradation of retinoblastoma RB1 protein. Inhibits DAXX-mediated apoptosis by inducing its ubiquitination and degradation. Component of the TRIM28/KAP1-MDM2-p53/TP53 complex involved in stabilizing p53/TP53. Also component of the TRIM28/KAP1-ERBB4-MDM2 complex which links growth factor and DNA damage response pathways. Mediates ubiquitination and subsequent proteasome degradation of DYRK2 in nucleus. Ubiquitinates IGF1R and SNAI1 and promotes them to proteasomal degradation. Ubiquitinates DCX, leading to DCX degradation and reduction of the dendritic spine density of olfactory bulb granule cells (By similarity). Ubiquitinates DLG4, leading to proteasomal degradation of DLG4 which is required for AMPA receptor endocytosis (By similarity).
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