Polyclonal antibody to Phospho-LMNA-S22
LMNA antibody; CDCD1 antibody; CDDC antibody; CMD1A antibody; CMT2B1 antibody; EMD2 antibody; FPL antibody; FPLD antibody; FPLD2 antibody; HGPS antibody; IDC antibody; LDP1 antibody; LFP antibody; LGMD1B antibody; LMN1 antibody; LMNC antibody; LMNL1 antibody; MADA antibody; PRO1 antibody; lamin antibody
Antigen: A synthetic phosphorylated peptide around S22 of human LMNA (NP_005563.1).
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Store at -20℃. Avoid freeze / thaw cycles.
WB 1:500 - 1:2000
IP 1:50 - 1:100
Western blot - Phospho-LMNA-S22 pAb
Western blot analysis of extracts of various cell lines, using Phospho-LMNA-S22 antibody at 1:2000 dilution or LMNA antibody . HeLa cells were treated by Hydroxyurea (4 mM) at 37u2103 for 20 hours or treated by Paclitaxel (100 nM/ml) at 37u2103 for 20 hours. C6 cells were treated by Hydroxyurea (4 mM) at 37u2103 for 20 hours or treated by Paclitaxel (100 nM) at 37u2103 for 20 hours.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% BSA.Detection: ECL Enhanced Kit .Exposure time: 1s.
Immunoprecipitation - Phospho-LMNA-S22 pAb
Immunoprecipitation analysis of 200ug extracts of HeLa cells, using 3 ug Phospho-LMNA-S22 pAb . Western blot was performed from the immunoprecipitate using Phospho-LMNA-S22 pAb at a dilition of 1:1000. HeLa cells were treated by Paclitaxel (100 nM/ml) at 37u2103 for 20 hours.
Lamins are components of the nuclear lamina, a fibrous layer on the nucleoplasmic side of the inner nuclear membrane, which is thought to provide a framework for the nuclear envelope and may also interact with chromatin. Lamin A and C are present in equal amounts in the lamina of mammals. Plays an important role in nuclear assembly, chromatin organization, nuclear membrane and telomere dynamics. Required for normal development of peripheral nervous system and skeletal muscle and for muscle satellite cell proliferation. Required for osteoblastogenesis and bone formation. Also prevents fat infiltration of muscle and bone marrow, helping to maintain the volume and strength of skeletal muscle and bone. Required for cardiac homeostasis.; Prelamin-A/C can accelerate smooth muscle cell senescence. It acts to disrupt mitosis and induce DNA damage in vascular smooth muscle cells (VSMCs), leading to mitotic failure, genomic instability, and premature senescence.
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