Polyclonal antibody to Phospho-Lck-Y416/FYN-Y416
Antigen: A phospho specific peptide corresponding to residues surrounding Y416 of human Lck and Y416 of human FYN
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Store at -20℃. Avoid freeze / thaw cycles.
WB 1:500 - 1:2000
IP 1:50 - 1:100
Western blot - Phospho-Lck-Y416/FYN-Y416 pAb
Western blot analysis of extracts of Jurkat cells, using Phospho-Lck-Y416/FYN-Y416 antibody at 1:1000 dilution. Jurkat cells were treated by UV for 15-30 minutes or treated by Hydrogen Peroxide (2mM) for 2 minutes after serum-starvation overnight.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% BSA.
Immunoprecipitation - Phospho-Lck-Y416/FYN-Y416 pAb
Immunoprecipitation analysis of 200ug extracts of Jurkat cells, using 3 ug Phospho-Lck-Y416/FYN-Y416 pAb . Western blot was performed from the immunoprecipitate using Phospho-Lck-Y416/FYN-Y416 pAb at a dilition of 1:1000. Jurkat cells were treated by Hydrogen Peroxide (2 mM) at 37u2103 for 2 minutes after serum-starvation overnight.
Non-receptor tyrosine-protein kinase that plays an essential role in the selection and maturation of developing T-cells in the thymus and in the function of mature T-cells. Plays a key role in T-cell antigen receptor (TCR)-linked signal transduction pathways. Constitutively associated with the cytoplasmic portions of the CD4 and CD8 surface receptors. Association of the TCR with a peptide antigen-bound MHC complex facilitates the interaction of CD4 and CD8 with MHC class II and class I molecules, respectively, thereby recruiting the associated LCK protein to the vicinity of the TCR/CD3 complex. LCK then phosphorylates tyrosine residues within the immunoreceptor tyrosine-based activation motifs (ITAM) of the cytoplasmic tails of the TCR-gamma chains and CD3 subunits, initiating the TCR/CD3 signaling pathway. Once stimulated, the TCR recruits the tyrosine kinase ZAP70, that becomes phosphorylated and activated by LCK. Following this, a large number of signaling molecules are recruited, ultimately leading to lymphokine production. LCK also contributes to signaling by other receptor molecules. Associates directly with the cytoplasmic tail of CD2, which leads to hyperphosphorylation and activation of LCK. Also plays a role in the IL2 receptor-linked signaling pathway that controls the T-cell proliferative response. Binding of IL2 to its receptor results in increased activity of LCK. Is expressed at all stages of thymocyte development and is required for the regulation of maturation events that are governed by both pre-TCR and mature alpha beta TCR. Phosphorylates other substrates including RUNX3, PTK2B/PYK2, the microtubule-associated protein MAPT, RHOH or TYROBP. Interacts with FYB2.
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