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Product Name
Phospho-LAT-Y171 pAb
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Description
Polyclonal antibody to Phospho-LAT-Y171
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Tested applications
WB
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Species reactivity
Human
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Alternative names
LAT antibody; LAT1 antibody; pp36 antibody; IMD52 antibody; linker for activation of T-cells antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: A synthetic phosphorylated peptide around Y171 of human LAT (NP_001014987.1).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
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Validations
Western blot - Phospho-LAT-Y171 pAb
Western blot analysis of extracts of Jurkat cells, using Phospho-LAT-Y171 antibody at 1:1000 dilution. Jurkat cells were treated by Hydrogen Peroxide (2mM) for 2 minutes after serum-starvation overnight or treated by CD-3 (10 ug/mL) for 2 minutes after serum-starvation overnight.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% BSA.Detection: ECL Basic Kit .Exposure time: 5s.
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Background
Required for TCR (T-cell antigen receptor)- and pre-TCR-mediated signaling, both in mature T-cells and during their development. Involved in FCGR3 (low affinity immunoglobulin gamma Fc region receptor III)-mediated signaling in natural killer cells and FCER1 (high affinity immunoglobulin epsilon receptor)-mediated signaling in mast cells. Couples activation of these receptors and their associated kinases with distal intracellular events such as mobilization of intracellular calcium stores, PKC activation, MAPK activation or cytoskeletal reorganization through the recruitment of PLCG1, GRB2, GRAP2, and other signaling molecules.
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References
Related Products / Services
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"