NovoPro
Cart 2
  • Phospho-LAT-Y171 pAb

Phospho-LAT-Y171 pAb

Cat.#: 166528

Special Price 232.3 USD

Availability: In Stock
- +

Add to cart to get an online quotation

Product Information

  • Product Name
    Phospho-LAT-Y171 pAb
  • Documents
  • Description
    Polyclonal antibody to Phospho-LAT-Y171
  • Tested applications
    WB
  • Species reactivity
    Human
  • Alternative names
    LAT antibody; LAT1 antibody; pp36 antibody; IMD52 antibody; linker for activation of T-cells antibody
  • Isotype
    Rabbit IgG
  • Preparation
    Antigen: A synthetic phosphorylated peptide around Y171 of human LAT (NP_001014987.1).
  • Clonality
    Polyclonal
  • Formulation
    PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
  • Storage instructions
    Store at -20℃. Avoid freeze / thaw cycles.
  • Applications
    WB 1:500 - 1:2000
  • Validations

    Western blot - Phospho-LAT-Y171 pAb

    Western blot - Phospho-LAT-Y171 pAb

    Western blot analysis of extracts of Jurkat cells, using Phospho-LAT-Y171 antibody at 1:1000 dilution. Jurkat cells were treated by Hydrogen Peroxide (2mM) for 2 minutes after serum-starvation overnight or treated by CD-3 (10 ug/mL) for 2 minutes after serum-starvation overnight.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% BSA.Detection: ECL Basic Kit .Exposure time: 5s.

  • Background
    Required for TCR (T-cell antigen receptor)- and pre-TCR-mediated signaling, both in mature T-cells and during their development. Involved in FCGR3 (low affinity immunoglobulin gamma Fc region receptor III)-mediated signaling in natural killer cells and FCER1 (high affinity immunoglobulin epsilon receptor)-mediated signaling in mast cells. Couples activation of these receptors and their associated kinases with distal intracellular events such as mobilization of intracellular calcium stores, PKC activation, MAPK activation or cytoskeletal reorganization through the recruitment of PLCG1, GRB2, GRAP2, and other signaling molecules.
  • References

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"