Cart 2
  • Phospho-JAK2-Y221 pAb
  • Phospho-JAK2-Y221 pAb

Phospho-JAK2-Y221 pAb

Cat.#: 166417

Special Price 232.3 USD

Availability: In Stock
- +

Add to cart to get an online quotation

Product Information

  • Product Name
    Phospho-JAK2-Y221 pAb
  • Documents
  • Description
    Polyclonal antibody to Phospho-JAK2-Y221
  • Tested applications
    WB, IHC
  • Species reactivity
    Human, Mouse, Rat
  • Alternative names
    JAK2 antibody; JTK10 antibody; THCYT3 antibody; Janus kinase 2 antibody
  • Isotype
    Rabbit IgG
  • Preparation
    Antigen: A phospho specific peptide corresponding to residues surrounding Y221 of human JAK2
  • Clonality
  • Formulation
    PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
  • Storage instructions
    Store at -20℃. Avoid freeze / thaw cycles.
  • Applications
    WB 1:500 - 1:2000
    IHC 1:50 - 1:100
  • Validations

    Western blot - Phospho-JAK2-Y221 pAb

    Western blot - Phospho-JAK2-Y221 pAb

    Western blot analysis of extracts of K562 cells, using Phospho-JAK2-Y221 antibody at 1:1000 dilution. K562 cell lysate were treated by CIP (20ul CIP for each 400ul cell lysate) at 37u2103 for 1 hour .Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% BSA.Detection: ECL Basic Kit .Exposure time: 30s.

    Immunohistochemistry - Phospho-JAK2-Y221 pAb

    Immunohistochemistry - Phospho-JAK2-Y221 pAb

    Immunohistochemistry of paraffin-embedded human breast carcinoma tissue, using Phospho-JAK2-Y221 antibody .

  • Background
    Non-receptor tyrosine kinase involved in various processes such as cell growth, development, differentiation or histone modifications. Mediates essential signaling events in both innate and adaptive immunity. In the cytoplasm, plays a pivotal role in signal transduction via its association with type I receptors such as growth hormone (GHR), prolactin (PRLR), leptin (LEPR), erythropoietin (EPOR), thrombopoietin (THPO); or type II receptors including IFN-alpha, IFN-beta, IFN-gamma and multiple interleukins. Following ligand-binding to cell surface receptors, phosphorylates specific tyrosine residues on the cytoplasmic tails of the receptor, creating docking sites for STATs proteins. Subsequently, phosphorylates the STATs proteins once they are recruited to the receptor. Phosphorylated STATs then form homodimer or heterodimers and translocate to the nucleus to activate gene transcription. For example, cell stimulation with erythropoietin (EPO) during erythropoiesis leads to JAK2 autophosphorylation, activation, and its association with erythropoietin receptor (EPOR) that becomes phosphorylated in its cytoplasmic domain. Then, STAT5 (STAT5A or STAT5B) is recruited, phosphorylated and activated by JAK2. Once activated, dimerized STAT5 translocates into the nucleus and promotes the transcription of several essential genes involved in the modulation of erythropoiesis. Part of a signaling cascade that is activated by increased cellular retinol and that leads to the activation of STAT5 (STAT5A or STAT5B). In addition, JAK2 mediates angiotensin-2-induced ARHGEF1 phosphorylation. Plays a role in cell cycle by phosphorylating CDKN1B. Cooperates with TEC through reciprocal phosphorylation to mediate cytokine-driven activation of FOS transcription. In the nucleus, plays a key role in chromatin by specifically mediating phosphorylation of 'Tyr-41' of histone H3 (H3Y41ph), a specific tag that promotes exclusion of CBX5 (HP1 alpha) from chromatin.
  • References