-
Product Name
Phospho-Jak1-Y1022/1023 pAb
- Documents
-
Description
Polyclonal antibody to Phospho-Jak1-Y1022/1023
-
Tested applications
WB
-
Species reactivity
Human
-
Alternative names
JAK1 antibody; JAK1A antibody; JAK1B antibody; JTK3 antibody; Janus kinase 1 antibody
-
Isotype
Rabbit IgG
-
Preparation
Antigen: A phospho specific peptide corresponding to residues surrounding Y1022/1023 of human JAK1
-
Clonality
Polyclonal
-
Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
-
Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
-
Applications
WB 1:500 - 1:2000
-
Validations
Western blot - Phospho-Jak1-Y1022/1023 pAb
Western blot analysis of extracts of HT-29 cells, using Phospho-Jak1-Y1022/1023 pAb at 1:2000 dilution. HT-29 cells were treated by UV at room temperature for 15-30 minutes.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% BSA.Detection: ECL Basic Kit .Exposure time: 5s.
-
Background
Tyrosine kinase of the non-receptor type, involved in the IFN-alpha/beta/gamma signal pathway. Kinase partner for the interleukin (IL)-2 receptor as well as interleukin (IL)-10 receptor.
-
References
Related Products / Services
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"