Polyclonal antibody to Phospho-Jak1-Y1022/1023
JAK1 antibody; JAK1A antibody; JAK1B antibody; JTK3 antibody; Janus kinase 1 antibody
Antigen: A phospho specific peptide corresponding to residues surrounding Y1022/1023 of human JAK1
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Store at -20℃. Avoid freeze / thaw cycles.
WB 1:500 - 1:2000
Western blot - Phospho-Jak1-Y1022/1023 pAb
Western blot analysis of extracts of HT-29 cells, using Phospho-Jak1-Y1022/1023 pAb at 1:2000 dilution. HT-29 cells were treated by UV at room temperature for 15-30 minutes.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% BSA.Detection: ECL Basic Kit .Exposure time: 5s.
Tyrosine kinase of the non-receptor type, involved in the IFN-alpha/beta/gamma signal pathway. Kinase partner for the interleukin (IL)-2 receptor as well as interleukin (IL)-10 receptor.
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