Phospho-Histone H3-T11 pAb
Polyclonal antibody to Phospho-Histone H3-T11
Human, Mouse, Rat
HIST1H3J antibody; H3/j antibody; H3FJ antibody; histone H3.1 antibody
Antigen: A phospho specific peptide corresponding to residues surrounding T11 of human HIST1H3J
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Store at -20℃. Avoid freeze / thaw cycles.
WB 1:500 - 1:2000
Western blot - Phospho-Histone H3-T11 pAb
Western blot analysis of extracts from U-87MG cells, using Phospho-Histone H3-T11 Antibody .U-87MG cells were treated by EGF (100 ng/ml) at 37u2103 for 30 minutes after serum-starvation overnight.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% BSA.
Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
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