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  • Phospho-Histone H3-S28 pAb
  • Phospho-Histone H3-S28 pAb
  • Phospho-Histone H3-S28 pAb
  • Phospho-Histone H3-S28 pAb

Phospho-Histone H3-S28 pAb

Cat.#: 166536

Special Price 232.3 USD

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Product Information

  • Product Name
    Phospho-Histone H3-S28 pAb
  • Documents
  • Description
    Polyclonal antibody to Phospho-Histone H3-S28
  • Tested applications
    WB, IF
  • Species reactivity
    Human, Mouse
  • Alternative names
    HIST1H3A antibody; H3/A antibody; H3FA antibody; H3.4 antibody; H3/g antibody; H3FT antibody; H3t antibody; histone H3.1 antibody
  • Isotype
    Rabbit IgG
  • Preparation
    Antigen: A synthetic phosphorylated peptide around S28 of human Histone H3 (NP_003520.1).
  • Clonality
    Polyclonal
  • Formulation
    PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
  • Storage instructions
    Store at -20℃. Avoid freeze / thaw cycles.
  • Applications
    WB 1:500 - 1:2000
    IF 1:50 - 1:200
  • Validations

    Western blot - Phospho-Histone H3-S28 pAb

    Western blot - Phospho-Histone H3-S28 pAb

    Western blot analysis of extracts of various cell lines, using Phospho-Histone H3-S28 antibody at 1:1000 dilution. Both HeLa cells and NIH/3T3 cells were treated by Calyculin A (100 nM) at 37u2103 for 30 minutes after serum-starvation overnight.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% BSA.Detection: ECL Basic Kit .Exposure time: 1s.

    Immunofluorescence - Phospho-Histone H3-S28 pAb

    Immunofluorescence - Phospho-Histone H3-S28 pAb

    Immunofluorescence analysis of H9C2 cells using Phospho-Histone H3-S28 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.

    Immunofluorescence - Phospho-Histone H3-S28 pAb

    Immunofluorescence - Phospho-Histone H3-S28 pAb

    Immunofluorescence analysis of NIH/3T3 cells using Phospho-Histone H3-S28 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.

    Immunofluorescence - Phospho-Histone H3-S28 pAb

    Immunofluorescence - Phospho-Histone H3-S28 pAb

    Immunofluorescence analysis of U2OS cells using Phospho-Histone H3-S28 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.

  • Background
    Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
  • References

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"