-
Product Name
Phospho-Histone H3-S28 pAb
- Documents
-
Description
Polyclonal antibody to Phospho-Histone H3-S28
-
Tested applications
WB, IF
-
Species reactivity
Human, Mouse
-
Alternative names
HIST1H3A antibody; H3/A antibody; H3FA antibody; H3.4 antibody; H3/g antibody; H3FT antibody; H3t antibody; histone H3.1 antibody
-
Isotype
Rabbit IgG
-
Preparation
Antigen: A synthetic phosphorylated peptide around S28 of human Histone H3 (NP_003520.1).
-
Clonality
Polyclonal
-
Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
-
Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
-
Applications
WB 1:500 - 1:2000
IF 1:50 - 1:200 -
Validations
Western blot - Phospho-Histone H3-S28 pAb
Western blot analysis of extracts of various cell lines, using Phospho-Histone H3-S28 antibody at 1:1000 dilution. Both HeLa cells and NIH/3T3 cells were treated by Calyculin A (100 nM) at 37u2103 for 30 minutes after serum-starvation overnight.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% BSA.Detection: ECL Basic Kit .Exposure time: 1s.
Immunofluorescence - Phospho-Histone H3-S28 pAb
Immunofluorescence analysis of H9C2 cells using Phospho-Histone H3-S28 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence - Phospho-Histone H3-S28 pAb
Immunofluorescence analysis of NIH/3T3 cells using Phospho-Histone H3-S28 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence - Phospho-Histone H3-S28 pAb
Immunofluorescence analysis of U2OS cells using Phospho-Histone H3-S28 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
-
Background
Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
-
References
Related Products / Services
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"