NovoPro
Cart 2
  • Phospho-Histone H3-S10/T11 pAb
  • Phospho-Histone H3-S10/T11 pAb
  • Phospho-Histone H3-S10/T11 pAb
  • Phospho-Histone H3-S10/T11 pAb
  • Phospho-Histone H3-S10/T11 pAb
  • Phospho-Histone H3-S10/T11 pAb
  • Phospho-Histone H3-S10/T11 pAb
  • Phospho-Histone H3-S10/T11 pAb
  • Phospho-Histone H3-S10/T11 pAb

Phospho-Histone H3-S10/T11 pAb

Cat.#: 166551

Special Price 232.3 USD

Availability: In Stock
- +

Add to cart to get an online quotation

Product Information

  • Product Name
    Phospho-Histone H3-S10/T11 pAb
  • Documents
  • Description
    Polyclonal antibody to Phospho-Histone H3-S10/T11
  • Tested applications
    WB, IHC, IF
  • Species reactivity
    Human, Mouse, Rat
  • Alternative names
    antibody
  • Isotype
    Rabbit IgG
  • Preparation
    Antigen: A phospho specific peptide corresponding to residues surrounding S10/T11 of human Histone H3.
  • Clonality
    Polyclonal
  • Formulation
    PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
  • Storage instructions
    Store at -20℃. Avoid freeze / thaw cycles.
  • Applications
    WB 1:500 - 1:2000
    IHC 1:50 - 1:200
    IF 1:50 - 1:200
  • Validations

    Western blot - Phospho-Histone H3-S10/T11 pAb

    Western blot - Phospho-Histone H3-S10/T11 pAb

    Western blot analysis of extracts of various cell lines, using Phospho-Histone H3-S10/T11 antibody at 1:1000 dilution. Both NIH/3T3 cells and Hela cells were treated by CIP(20uL/400ul) at 37u2103 for 1 hour and treated by nocodazole (50 ng/ml) at 37u2103 for 20 hours.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% BSA.Detection: ECL Basic Kit .Exposure time: 1s.

    Western blot - Phospho-Histone H3-S10/T11 pAb

    Western blot - Phospho-Histone H3-S10/T11 pAb

    Western blot analysis of extracts of NIH/3T3 cells, using phospho-STK4-T387 pAb at 1:1000 dilution or Histone H3 antibody .HeLa,NIH/3T3 and C6 cellls were treated by nocodazole (50 ng/ml) at 37u2103 for 20 hours.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% BSA.Detection: ECL Basic Kit .Exposure time: 1s.

    Immunohistochemistry - Phospho-Histone H3-S10/T11 pAb

    Immunohistochemistry - Phospho-Histone H3-S10/T11 pAb

    Immunohistochemistry of paraffin-embedded human lung cancer using Phospho-Histone H3-S10/T11 antibody at dilution of 1:100 (40x lens).

    Immunohistochemistry - Phospho-Histone H3-S10/T11 pAb

    Immunohistochemistry - Phospho-Histone H3-S10/T11 pAb

    Immunohistochemistry of paraffin-embedded rat liver using Phospho-Histone H3-S10/T11 antibody at dilution of 1:100 (40x lens).

    Immunohistochemistry - Phospho-Histone H3-S10/T11 pAb

    Immunohistochemistry - Phospho-Histone H3-S10/T11 pAb

    Immunohistochemistry of paraffin-embedded mouse kidney using Phospho-Histone H3-S10/T11 antibody at dilution of 1:100 (40x lens).

    Immunofluorescence - Phospho-Histone H3-S10/T11 pAb

    Immunofluorescence - Phospho-Histone H3-S10/T11 pAb

    Immunofluorescence analysis of C6 cells using Phospho-Histone H3-S10/T11 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.

    Immunofluorescence - Phospho-Histone H3-S10/T11 pAb

    Immunofluorescence - Phospho-Histone H3-S10/T11 pAb

    Immunofluorescence analysis of NIH-3T3 cells using Phospho-Histone H3-S10/T11 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.

    Immunofluorescence - Phospho-Histone H3-S10/T11 pAb

    Immunofluorescence - Phospho-Histone H3-S10/T11 pAb

    Immunofluorescence analysis of C6 cells using Phospho-Histone H3-S10/T11 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.

    Immunofluorescence - Phospho-Histone H3-S10/T11 pAb

    Immunofluorescence - Phospho-Histone H3-S10/T11 pAb

    Immunofluorescence analysis of NIH-3T3 cells using Phospho-Histone H3-S10/T11 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.

  • Background
    Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
  • References

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"