Phospho-Histone H3-S10 pAb
Polyclonal antibody to Phospho-Histone H3-S10
HIST3H3 antibody; H3.4 antibody; H3/g antibody; H3FT antibody; H3t antibody; histone H3.1t antibody
Antigen: A synthetic phosphorylated peptide around S10 of human Histone H3 (NP_003484.1).
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Store at -20℃. Avoid freeze / thaw cycles.
WB 1:500 - 1:2000
Western blot - Phospho-Histone H3-S10 pAb
Western blot analysis of extracts of HeLa cells, using Phospho-Histone H3-S10 antibody at 1:1000 dilution. HeLa cells were treated by Calyculin A (100 nM) at 37u2103 for 30 minutes after serum-starvation overnight.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% BSA.Detection: ECL Basic Kit .Exposure time: 180s.
Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
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