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Product Name
Phospho-Histone H3-S10 mAb
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Description
Monoclonal antibody to Phospho-Histone H3-S10
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Tested applications
WB, IHC, IF, IP
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Species reactivity
Human, Mouse, Rat
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Alternative names
HIST1H3A antibody; H3/A antibody; H3FA antibody; histone H3.1 antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: A phospho specific peptide corresponding to residues surrounding S10 of human Histone H3
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Clonality
Monoclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
IP 1:20 - 1:50 -
Validations
Western blot - Phospho-Histone H3-S10 mAb
Western blot analysis of extracts of various cells, using Phospho-Histone H3-S10 antibody .Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% BSA.
Immunohistochemistry - Phospho-Histone H3-S10 mAb
Immunohistochemistry of paraffin-embedded mouse testis using Phospho-Histone H3-S10 antibody at dilution of 1:100 (40x lens).
Immunofluorescence - Phospho-Histone H3-S10 mAb
Immunofluorescence analysis of HepG2 cells using Phospho-Histone H3-S10 antibody .
Immunofluorescence - Phospho-Histone H3-S10 mAb
Immunofluorescence analysis of HeLa cells using Phospho-Histone H3-S10 antibody .
Immunofluorescence - Phospho-Histone H3-S10 mAb
Immunofluorescence analysis of Ags cells using Phospho-Histone H3-S10 antibody .
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Background
Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
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References
Related Products / Services
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"