Polyclonal antibody to Phospho-GRIA1-S845
Human, Mouse, Rat
GRIA1 antibody; GLUH1 antibody; GLUR1 antibody; GLURA antibody; GluA1 antibody; HBGR1 antibody; glutamate receptor 1 antibody
Antigen: A phospho specific peptide corresponding to residues surrounding S845 of human GRIA1
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Store at -20℃. Avoid freeze / thaw cycles.
WB 1:500 - 1:2000
Western blot - Phospho-GRIA1-S845 pAb
Western blot analysis of extracts of SH-SY5Y cells, using Phospho-GRIA1-S845 antibody at 1:1000 dilution or GRIA1 antibody . SH-SY5Y cells were treated by CIP(20uL/400ul) at 37u2103 for 1 hour.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% BSA.Detection: ECL Basic Kit .Exposure time: 15s.
Ionotropic glutamate receptor. L-glutamate acts as an excitatory neurotransmitter at many synapses in the central nervous system. Binding of the excitatory neurotransmitter L-glutamate induces a conformation change, leading to the opening of the cation channel, and thereby converts the chemical signal to an electrical impulse. The receptor then desensitizes rapidly and enters a transient inactive state, characterized by the presence of bound agonist. In the presence of CACNG4 or CACNG7 or CACNG8, shows resensitization which is characterized by a delayed accumulation of current flux upon continued application of glutamate.
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