Polyclonal antibody to Phospho-FOXO3-S253
WB, IHC, IF
Human, Mouse, Rat
FOXO3 antibody; AF6q21 antibody; FKHRL1 antibody; FKHRL1P2 antibody; FOXO2 antibody; FOXO3A antibody; forkhead box O3 antibody
Antigen: A phospho specific peptide corresponding to residues surrounding S253 of human FOXO3
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Store at -20℃. Avoid freeze / thaw cycles.
WB 1:500 - 1:1000
IHC 1:50 - 1:100
IF 1:50 - 1:200
Western blot - Phospho-FOXO3-S253 pAb
Western blot analysis of extracts of Untreated and treated 293 cell line, using Phospho-FOXO3-S253 antibody .Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% BSA.
Transcriptional activator which triggers apoptosis in the absence of survival factors, including neuronal cell death upon oxidative stress. Recognizes and binds to the DNA sequence 5'-[AG]TAAA[TC]A-3'. Participates in post-transcriptional regulation of MYC: following phosphorylation by MAPKAPK5, promotes induction of miR-34b and miR-34c expression, 2 post-transcriptional regulators of MYC that bind to the 3'UTR of MYC transcript and prevent its translation. In response to metabolic stress, translocates into the mitochondria where it promotes mtDNA transcription.
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