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  • Phospho-ERK1-T202/Y204 + ERK2-T185/Y187 pAb
  • Phospho-ERK1-T202/Y204 + ERK2-T185/Y187 pAb
  • Phospho-ERK1-T202/Y204 + ERK2-T185/Y187 pAb
  • Phospho-ERK1-T202/Y204 + ERK2-T185/Y187 pAb
  • Phospho-ERK1-T202/Y204 + ERK2-T185/Y187 pAb
  • Phospho-ERK1-T202/Y204 + ERK2-T185/Y187 pAb
  • Phospho-ERK1-T202/Y204 + ERK2-T185/Y187 pAb
  • Phospho-ERK1-T202/Y204 + ERK2-T185/Y187 pAb
  • Phospho-ERK1-T202/Y204 + ERK2-T185/Y187 pAb
  • Phospho-ERK1-T202/Y204 + ERK2-T185/Y187 pAb
  • Phospho-ERK1-T202/Y204 + ERK2-T185/Y187 pAb
  • Phospho-ERK1-T202/Y204 + ERK2-T185/Y187 pAb
  • Phospho-ERK1-T202/Y204 + ERK2-T185/Y187 pAb
  • Phospho-ERK1-T202/Y204 + ERK2-T185/Y187 pAb
  • Phospho-ERK1-T202/Y204 + ERK2-T185/Y187 pAb
  • Phospho-ERK1-T202/Y204 + ERK2-T185/Y187 pAb
  • Phospho-ERK1-T202/Y204 + ERK2-T185/Y187 pAb

Phospho-ERK1-T202/Y204 + ERK2-T185/Y187 pAb

Cat.#: 166458

Special Price 232.3 USD

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Product Information

  • Product Name
    Phospho-ERK1-T202/Y204 + ERK2-T185/Y187 pAb
  • Documents
  • Description
    Polyclonal antibody to Phospho-ERK1-T202/Y204 + ERK2-T185/Y187
  • Tested applications
    WB, IHC, IP
  • Species reactivity
    Human, Mouse, Rat
  • Alternative names
    antibody
  • Isotype
    Rabbit IgG
  • Preparation
    Antigen: A synthetic phosphorylated peptide around T185/Y187 of human ERK2 (NP_002736.3).
  • Clonality
    Polyclonal
  • Formulation
    PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
  • Storage instructions
    Store at -20℃. Avoid freeze / thaw cycles.
  • Applications
    WB 1:500 - 1:2000
    IHC 1:50 - 1:100
    IP 1:50 - 1:100
  • Validations

    Western blot - Phospho-ERK1-T202/Y204 + ERK2-T185/Y187 pAb

    Western blot - Phospho-ERK1-T202/Y204 + ERK2-T185/Y187 pAb

    Western blot analysis of extracts of various cell lines, using Phospho-ERK1-T202/Y204 + ERK2-T185/Y187 antibody at 1:5000 dilution or ERK1/2 antibody .293T cells and C6 cells were treated by ATP(5 mM) at 30u2103 for 1 hour. NIH/3T3 cells were treated by PMA/TPA (200 nM) at 37u2103 for 30 minutes after serum-starvation overnight.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% BSA.Detection: ECL Basic Kit .Exposure time: 10s.

    Immunohistochemistry - Phospho-ERK1-T202/Y204 + ERK2-T185/Y187 pAb

    Immunohistochemistry - Phospho-ERK1-T202/Y204 + ERK2-T185/Y187 pAb

    Immunohistochemistry of paraffin-embedded rat lung using Phospho-ERK1-T202-Y204 + ERK2-T185-Y187 antibody at dilution of 1:100 (40x lens).

    Immunohistochemistry - Phospho-ERK1-T202/Y204 + ERK2-T185/Y187 pAb

    Immunohistochemistry - Phospho-ERK1-T202/Y204 + ERK2-T185/Y187 pAb

    Immunohistochemistry of paraffin-embedded rat testis using Phospho-ERK1-T202-Y204 + ERK2-T185-Y187 antibody at dilution of 1:100 (40x lens).

    Immunohistochemistry - Phospho-ERK1-T202/Y204 + ERK2-T185/Y187 pAb

    Immunohistochemistry - Phospho-ERK1-T202/Y204 + ERK2-T185/Y187 pAb

    Immunohistochemistry of paraffin-embedded rat brain using Phospho-ERK1-T202-Y204 + ERK2-T185-Y187 antibody at dilution of 1:100 (40x lens).

    Immunohistochemistry - Phospho-ERK1-T202/Y204 + ERK2-T185/Y187 pAb

    Immunohistochemistry - Phospho-ERK1-T202/Y204 + ERK2-T185/Y187 pAb

    Immunohistochemistry of paraffin-embedded rat spleen using Phospho-ERK1-T202-Y204 + ERK2-T185-Y187 antibody at dilution of 1:100 (40x lens).

    Immunohistochemistry - Phospho-ERK1-T202/Y204 + ERK2-T185/Y187 pAb

    Immunohistochemistry - Phospho-ERK1-T202/Y204 + ERK2-T185/Y187 pAb

    Immunohistochemistry of paraffin-embedded rat heart using Phospho-ERK1-T202-Y204 + ERK2-T185-Y187 antibody at dilution of 1:100 (40x lens).

    Immunohistochemistry - Phospho-ERK1-T202/Y204 + ERK2-T185/Y187 pAb

    Immunohistochemistry - Phospho-ERK1-T202/Y204 + ERK2-T185/Y187 pAb

    Immunohistochemistry of paraffin-embedded human tonsil using Phospho-ERK1-T202-Y204 + ERK2-T185-Y187 antibody at dilution of 1:100 (40x lens).

    Immunohistochemistry - Phospho-ERK1-T202/Y204 + ERK2-T185/Y187 pAb

    Immunohistochemistry - Phospho-ERK1-T202/Y204 + ERK2-T185/Y187 pAb

    Immunohistochemistry of paraffin-embedded human breast cancer using Phospho-ERK1-T202-Y204 + ERK2-T185-Y187 antibody at dilution of 1:100 (40x lens).

    Immunohistochemistry - Phospho-ERK1-T202/Y204 + ERK2-T185/Y187 pAb

    Immunohistochemistry - Phospho-ERK1-T202/Y204 + ERK2-T185/Y187 pAb

    Immunohistochemistry of paraffin-embedded human gastric cancer using Phospho-ERK1-T202-Y204 + ERK2-T185-Y187 antibody at dilution of 1:100 (40x lens).

    Immunohistochemistry - Phospho-ERK1-T202/Y204 + ERK2-T185/Y187 pAb

    Immunohistochemistry - Phospho-ERK1-T202/Y204 + ERK2-T185/Y187 pAb

    Immunohistochemistry of paraffin-embedded human uterine cancer using Phospho-ERK1-T202-Y204 + ERK2-T185-Y187 antibody at dilution of 1:100 (40x lens).

    Immunohistochemistry - Phospho-ERK1-T202/Y204 + ERK2-T185/Y187 pAb

    Immunohistochemistry - Phospho-ERK1-T202/Y204 + ERK2-T185/Y187 pAb

    Immunohistochemistry of paraffin-embedded mouse liver using Phospho-ERK1-T202-Y204 + ERK2-T185-Y187 antibody at dilution of 1:100 (40x lens).

    Immunohistochemistry - Phospho-ERK1-T202/Y204 + ERK2-T185/Y187 pAb

    Immunohistochemistry - Phospho-ERK1-T202/Y204 + ERK2-T185/Y187 pAb

    Immunohistochemistry of paraffin-embedded mouse testis using Phospho-ERK1-T202-Y204 + ERK2-T185-Y187 antibody at dilution of 1:100 (40x lens).

    Immunohistochemistry - Phospho-ERK1-T202/Y204 + ERK2-T185/Y187 pAb

    Immunohistochemistry - Phospho-ERK1-T202/Y204 + ERK2-T185/Y187 pAb

    Immunohistochemistry of paraffin-embedded mouse brain using Phospho-ERK1-T202-Y204 + ERK2-T185-Y187 antibody at dilution of 1:100 (40x lens).

    Immunohistochemistry - Phospho-ERK1-T202/Y204 + ERK2-T185/Y187 pAb

    Immunohistochemistry - Phospho-ERK1-T202/Y204 + ERK2-T185/Y187 pAb

    Immunohistochemistry of paraffin-embedded mouse spleen using Phospho-ERK1-T202-Y204 + ERK2-T185-Y187 antibody at dilution of 1:100 (40x lens).

    Immunohistochemistry - Phospho-ERK1-T202/Y204 + ERK2-T185/Y187 pAb

    Immunohistochemistry - Phospho-ERK1-T202/Y204 + ERK2-T185/Y187 pAb

    Immunohistochemistry of paraffin-embedded mouse kidney using Phospho-ERK1-T202-Y204 + ERK2-T185-Y187 antibody at dilution of 1:100 (40x lens).

    Immunohistochemistry - Phospho-ERK1-T202/Y204 + ERK2-T185/Y187 pAb

    Immunohistochemistry - Phospho-ERK1-T202/Y204 + ERK2-T185/Y187 pAb

    Immunohistochemistry of paraffin-embedded mouse heart using Phospho-ERK1-T202-Y204 + ERK2-T185-Y187 antibody at dilution of 1:100 (40x lens).

    Immunoprecipitation - Phospho-ERK1-T202/Y204 + ERK2-T185/Y187 pAb

    Immunoprecipitation - Phospho-ERK1-T202/Y204 + ERK2-T185/Y187 pAb

    Immunoprecipitation analysis of 200ug extracts of Jurkat cells, using 3 ug Phospho-ERK1-T202/Y204 + ERK2-T185/Y187 pAb . Western blot was performed from the immunoprecipitate using Phospho-ERK1-T202/Y204 + ERK2-T185/Y187 pAb at a dilition of 1:1000. Jurkat cells were treated by PMA/TPA (200 nM) at 37u2103 for 10 minutes.

  • Background
    Serine/threonine kinase which acts as an essential component of the MAP kinase signal transduction pathway. MAPK1/ERK2 and MAPK3/ERK1 are the 2 MAPKs which play an important role in the MAPK/ERK cascade. They participate also in a signaling cascade initiated by activated KIT and KITLG/SCF. Depending on the cellular context, the MAPK/ERK cascade mediates diverse biological functions such as cell growth, adhesion, survival and differentiation through the regulation of transcription, translation, cytoskeletal rearrangements. The MAPK/ERK cascade plays also a role in initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors. About 160 substrates have already been discovered for ERKs. Many of these substrates are localized in the nucleus, and seem to participate in the regulation of transcription upon stimulation. However, other substrates are found in the cytosol as well as in other cellular organelles, and those are responsible for processes such as translation, mitosis and apoptosis. Moreover, the MAPK/ERK cascade is also involved in the regulation of the endosomal dynamics, including lysosome processing and endosome cycling through the perinuclear recycling compartment (PNRC); as well as in the fragmentation of the Golgi apparatus during mitosis. The substrates include transcription factors (such as ATF2, BCL6, ELK1, ERF, FOS, HSF4 or SPZ1), cytoskeletal elements (such as CANX, CTTN, GJA1, MAP2, MAPT, PXN, SORBS3 or STMN1), regulators of apoptosis (such as BAD, BTG2, CASP9, DAPK1, IER3, MCL1 or PPARG), regulators of translation (such as EIF4EBP1) and a variety of other signaling-related molecules (like ARHGEF2, DCC, FRS2 or GRB10). Protein kinases (such as RAF1, RPS6KA1/RSK1, RPS6KA3/RSK2, RPS6KA2/RSK3, RPS6KA6/RSK4, SYK, MKNK1/MNK1, MKNK2/MNK2, RPS6KA5/MSK1, RPS6KA4/MSK2, MAPKAPK3 or MAPKAPK5) and phosphatases (such as DUSP1, DUSP4, DUSP6 or DUSP16) are other substrates which enable the propagation the MAPK/ERK signal to additional cytosolic and nuclear targets, thereby extending the specificity of the cascade. Mediates phosphorylation of TPR in respons to EGF stimulation. May play a role in the spindle assembly checkpoint. Phosphorylates PML and promotes its interaction with PIN1, leading to PML degradation. Phosphorylates CDK2AP2 (By similarity).; Acts as a transcriptional repressor. Binds to a [GC]AAA[GC] consensus sequence. Repress the expression of interferon gamma-induced genes. Seems to bind to the promoter of CCL5, DMP1, IFIH1, IFITM1, IRF7, IRF9, LAMP3, OAS1, OAS2, OAS3 and STAT1. Transcriptional activity is independent of kinase activity.
  • References

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"