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Product Name
Phospho-ELK1-S383 pAb
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Description
Polyclonal antibody to Phospho-ELK1-S383
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Tested applications
WB, IHC
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Species reactivity
Human, Mouse, Rat
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Alternative names
ELK1 antibody; ELK1, ETS transcription factor antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: A phospho specific peptide corresponding to residues surrounding S383 of human ELK1
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
IHC 1:50 - 1:100 -
Validations
Western blot - Phospho-ELK1-S383 pAb
Western blot analysis of extracts from HT29 cells, using Phospho-ELK1-S383 antibody .Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% BSA.
Immunohistochemistry - Phospho-ELK1-S383 pAb
Immunohistochemistry of paraffin-embedded human breast carcinoma using Phospho-ELK1-S383 antibody .
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Background
Transcription factor that binds to purine-rich DNA sequences. Forms a ternary complex with SRF and the ETS and SRF motifs of the serum response element (SRE) on the promoter region of immediate early genes such as FOS and IER2. Induces target gene transcription upon JNK-signaling pathway stimulation (By similarity).
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References
Related Products / Services
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"