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  • Phospho-EGFR (Tyr1016) Antibody

Phospho-EGFR (Tyr1016) Antibody

Cat.#: 134835

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Special Price 372.6 USD

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Product Information

  • Product Name
    Phospho-EGFR (Tyr1016) Antibody
  • Documents
  • Description
    Peptide affinitypurifiedRabbitPolyclonalAntibody(Pab)
  • Tested applications
    WB
  • Species reactivity
    Human, Mouse, Rat
  • Alternative names
    ERBB antibody; ERBB1 antibody; HER1 antibody; Epidermal growth factor receptor antibody; Proto-oncogene c-ErbB-1 antibody; Receptor tyrosine-protein kinase erbB-1 antibody; EGFR antibody; ERBB antibody; ERBB1 antibody; HER1 antibody
  • Isotype
    Rabbit IgG
  • Preparation
    Phospho-EGFR (Tyr1016) Antibody detects endogenous levels of EGFR only when phosphorylated at Tyrosine 1016
  • Clonality
    polyclonal
  • Formulation
    Peptide affinity purified.Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • Storage instructions
    Short term 4°C, long term aliquot and store at -20°C, avoid freeze thaw cycles. Store undiluted.
  • Applications
    WB: 1:500-1:2000
  • Validations

    Western blot analysis of EGFR phosphorylation expression in Serum treated HuvEc whole cell lysates,The lane on the left is treated with the antigen-specific peptide.

    Western blot analysis of EGFR phosphorylation expression in Serum treated HuvEc whole cell lysates,The lane on the left is treated with the antigen-specific peptide.

  • Background
    Receptor tyrosine kinase binding ligands of the EGF family and activating several signaling cascades to convert extracellular cues into appropriate cellular responses. Known ligands include EGF, TGFA/TGF-alpha, amphiregulin, epigen/EPGN, BTC/betacellulin, epiregulin/EREG and HBEGF/heparin-binding EGF. Ligand binding triggers receptor homo- and/or heterodimerization and autophosphorylation on key cytoplasmic residues. The phosphorylated receptor recruits adapter proteins like GRB2 which in turn activates complex downstream signaling cascades. Activates at least 4 major downstream signaling cascades including the RAS- RAF-MEK-ERK, PI3 kinase-AKT, PLCgamma-PKC and STATs modules. May also activate the NF-kappa-B signaling cascade. Also directly phosphorylates other proteins like RGS16, activating its GTPase activity and probably coupling the EGF receptor signaling to the G protein-coupled receptor signaling. Also phosphorylates MUC1 and increases its interaction with SRC and CTNNB1/beta-catenin.
  • References

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"