Phospho-EGFR (Tyr1016) Antibody
Human, Mouse, Rat
ERBB antibody; ERBB1 antibody; HER1 antibody; Epidermal growth factor receptor antibody; Proto-oncogene c-ErbB-1 antibody; Receptor tyrosine-protein kinase erbB-1 antibody; EGFR antibody; ERBB antibody; ERBB1 antibody; HER1 antibody
Phospho-EGFR (Tyr1016) Antibody detects endogenous levels of EGFR only when phosphorylated at Tyrosine 1016
Peptide affinity purified.Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Short term 4°C, long term aliquot and store at -20°C, avoid freeze thaw cycles. Store undiluted.
Western blot analysis of EGFR phosphorylation expression in Serum treated HuvEc whole cell lysates,The lane on the left is treated with the antigen-specific peptide.
Receptor tyrosine kinase binding ligands of the EGF family and activating several signaling cascades to convert extracellular cues into appropriate cellular responses. Known ligands include EGF, TGFA/TGF-alpha, amphiregulin, epigen/EPGN, BTC/betacellulin, epiregulin/EREG and HBEGF/heparin-binding EGF. Ligand binding triggers receptor homo- and/or heterodimerization and autophosphorylation on key cytoplasmic residues. The phosphorylated receptor recruits adapter proteins like GRB2 which in turn activates complex downstream signaling cascades. Activates at least 4 major downstream signaling cascades including the RAS- RAF-MEK-ERK, PI3 kinase-AKT, PLCgamma-PKC and STATs modules. May also activate the NF-kappa-B signaling cascade. Also directly phosphorylates other proteins like RGS16, activating its GTPase activity and probably coupling the EGF receptor signaling to the G protein-coupled receptor signaling. Also phosphorylates MUC1 and increases its interaction with SRC and CTNNB1/beta-catenin.
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