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Product Name
Phospho-CRKL-Y207 pAb
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Description
Polyclonal antibody to Phospho-CRKL-Y207
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Tested applications
WB, IHC
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Species reactivity
Human, Mouse, Rat
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Alternative names
CRKL antibody; crk-like protein antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: A synthetic phosphorylated peptide around Y207 of human CRKL (NP_005198.1).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
IHC 1:50 - 1:100 -
Validations
Western blot - Phospho-CRKL-Y207 pAb
Western blot analysis of extracts of K-562 cells, using Phospho-CRKL-Y207 antibody at 1:1000 dilution or CRKL antibody . K-562 cells were treated by CIP(20uL/400ul) at 37u2103 for 1 hour.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% BSA.Detection: ECL Basic Kit .Exposure time: 5s.
Immunohistochemistry - Phospho-CRKL-Y207 pAb
Immunohistochemistry of paraffin-embedded rat brain using Phospho-CRKL-Y207 antibody at dilution of 1:100 (40x lens).
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Background
May mediate the transduction of intracellular signals.
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References
Related Products / Services
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"