Polyclonal antibody to Phospho-CDK6-Y24
WB, IHC, IF
CDK6 antibody; MCPH12 antibody; PLSTIRE antibody; cyclin-dependent kinase 6 antibody
Antigen: A phospho specific peptide corresponding to residues surrounding Y24 of human CDK6
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Store at -20℃. Avoid freeze / thaw cycles.
WB 1:500 - 1:2000
IHC 1:50 - 1:100
IF 1:100 - 1:200
Western blot - Phospho-CDK6-Y24 pAb
Western blot analysis of extracts from 293 cells using Phospho-CDK6-Y24 antibody .Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% BSA.
Immunohistochemistry - Phospho-CDK6-Y24 pAb
Immunohistochemistry of paraffin-embedded human colon carcinoma using Phospho-CDK6-Y24 antibody .
Immunofluorescence - Phospho-CDK6-Y24 pAb
Immunofluorescence analysis of methanol-fixed HeLa cells using Phospho-CDK6-Y24 antibody .
Serine/threonine-protein kinase involved in the control of the cell cycle and differentiation; promotes G1/S transition. Phosphorylates pRB/RB1 and NPM1. Interacts with D-type G1 cyclins during interphase at G1 to form a pRB/RB1 kinase and controls the entrance into the cell cycle. Involved in initiation and maintenance of cell cycle exit during cell differentiation; prevents cell proliferation and regulates negatively cell differentiation, but is required for the proliferation of specific cell types (e.g. erythroid and hematopoietic cells). Essential for cell proliferation within the dentate gyrus of the hippocampus and the subventricular zone of the lateral ventricles. Required during thymocyte development. Promotes the production of newborn neurons, probably by modulating G1 length. Promotes, at least in astrocytes, changes in patterns of gene expression, changes in the actin cytoskeleton including loss of stress fibers, and enhanced motility during cell differentiation. Prevents myeloid differentiation by interfering with RUNX1 and reducing its transcription transactivation activity, but promotes proliferation of normal myeloid progenitors. Delays senescence. Promotes the proliferation of beta-cells in pancreatic islets of Langerhans. May play a role in the centrosome organization during the cell cycle phases.
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