Polyclonal antibody to Phospho-cdc25C-S216
CDC25C antibody; CDC25 antibody; PPP1R60 antibody; cell division cycle 25C antibody
Antigen: A synthetic phosphorylated peptide around S216 of human cdc25C (NP_001781.2).
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Store at -20℃. Avoid freeze / thaw cycles.
WB 1:500 - 1:2000
Western blot - Phospho-cdc25C-S216 pAb
Western blot analysis of extracts of HT-29 cells, using Phospho-cdc25C-S216 antibody .Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% BSA.
Functions as a dosage-dependent inducer in mitotic control. Tyrosine protein phosphatase required for progression of the cell cycle. When phosphorylated, highly effective in activating G2 cells into prophase. Directly dephosphorylates CDK1 and activates its kinase activity.
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