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Product Name
Phospho-cdc25C-S216 pAb
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Description
Polyclonal antibody to Phospho-cdc25C-S216
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Tested applications
WB
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Species reactivity
Human
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Alternative names
CDC25C antibody; CDC25 antibody; PPP1R60 antibody; cell division cycle 25C antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: A synthetic phosphorylated peptide around S216 of human cdc25C (NP_001781.2).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
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Validations
Western blot - Phospho-cdc25C-S216 pAb
Western blot analysis of extracts of HT-29 cells, using Phospho-cdc25C-S216 antibody .Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% BSA.
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Background
Functions as a dosage-dependent inducer in mitotic control. Tyrosine protein phosphatase required for progression of the cell cycle. When phosphorylated, highly effective in activating G2 cells into prophase. Directly dephosphorylates CDK1 and activates its kinase activity.
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References
Related Products / Services
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"